Status of sub-health and its influencing factors in community health care workers in Hongkou District, Shanghai / 上海预防医学

Objective To determine the sub-health status and influencing factors among medical staff in community health service centers, and to provide scientific evidence for intervention measures and strategies of health promotion. Methods A questionnaire survey was conducted in 692 community medical staff from 8 community health service centers in Hongkou District, Shanghai.Sub-health condition scale was used to identify the incidence rate of sub-health.Simple job stress questionnaire was used to identify the incidence rate of occupational stress.The correlations between sub-health status and demographic and sociological factors, living habits, occupational stress and other factors were determined by multivariate logistic regression analysis. Results The incidence rate of sub-health among targeted community medical staff was 71.4%.The following factors were shown to be related to the incidence of sub-health: women (OR=2.24, 95%CI: 1.42-3.55), per capita living area ≤ 30 m2(OR=2.19, 95%CI: 1.24-3.87), daily average sleep time < 7 hours (OR=1.64, 95%CI: 1.10-2.46), habits of drinking alcohol (OR=1.58, 95%CI: 1.04-2.40), frequent overtime work (OR=1.79, 95%CI: 1.12-2.86) and occupational stress (OR=1.73, 95%CI: 1.16-2.58). Conclusion The incidence rate of sub-health among medical staff in community health service centers is high, and unhealthy living habits and occupational stress are the influencing factors.

Effects of Action Observation Training on Motor Function of Upper Extremities after Stroke / 中国康复理论与实践

Objective:To explore the effects of action observation training based on mirror neuron system on motor function of upper extremities in stroke patients. Methods:From June, 2016 to June, 2017, 61 stroke patients were randomly assigned to control group (n = 30) and observation group (n = 31). They all accepted routine rehabilitation, and the observation group received action observation therapy in addition, for eight weeks. They were assessed with Fugl-Meyer Assessment-Upper Extremities, modified Ashworth Scale and modified Barthel Index before and after treatment. Results:All the indexes improved in both groups after treatment (|t| > 2.651, P < 0.05), and improved more in the observation group than in the control group (|t| > 2.048, P < 0.05). Conclusion:The action observation training may further improve the upper extremity motor function in stroke patients.

Effect analysis on remission policy for TB treatment fee in Tilanqiao community of Shanghai / 上海预防医学

Objective To analyze the change in cost reduction for tuberculosis (TB) patients in Tilanqiao community of Shanghai from 2006 to 2016,and to evaluate the implement effect of TB remission policy in Shanghai.Methods The data were collected on treatment costs for 309 TB patients who had completed treatment and participated in the cost remission reimbursement by outpatient registration.The trend of deductible expenses and the remission costs were analyzed by SPSS.Results During the implementation of the original remission policy(ORP),the deductible expense was 1 021.06±457.67 yuan.And it changed to 2 320.64±1 544.73yuan under the new remission policy (NRP),which had an increase of 1.27 times and the new remission fee ratio was 45.19%.The deductible expenses change curve shown a trend of rising.The new remission fee ratio was 27.62% in 2009 and increased to 46.62% in 2010,then became 51.05% in 2016.The new remission fee ratio for outpatients was significantly lower than that for the hospitalized.During the implementation of the ORP,the remission cost was 931.05±465.47 yuan,and it was 1 507.80±974.15 of the NRP,which increased 61.95%.The remission ratio was 17.55% of the ORP,when it was 15.91% of the NRP which decreased by 1.64 percent.The remission ratio curve showed a trend of downward.The remission ratio for outpatients was significantly higher than that for the hospitalized.Conclusion From 2006 to 2016,the implement effect of remission policy on treatment costs of tuberculosis had improved.However,it had not effectively reduced the disease burden of TB patients.So in the follow-up remission policy,we must strengthen improvement on the settings of deductible project and control disease treatment.

Relationship between children's motor coordination and their physical growth / 中华儿科杂志

<p><b>OBJECTIVE</b>To explore the relationship between children's motor coordination and their physical growth to provide clues for clarifying the causal relationship between them and to effectively enhance the children's physical growth.</p><p><b>METHOD</b>Ten kindergartens were selected randomly from district of Hongkou, Putuo and Pudong in Shanghai, and 1099 children were included in the analysis. The children's motor coordination was assessed using Developmental Coordination Disorder Questionnaire (DCDQ). The relationship between the children's motor coordination and their physical growth were analyzed by Structural Equation Model (SEM). The above relationship by different gender, age and BMI (overweight or not) were analyzed with multi-group SEM.</p><p><b>RESULTS</b>Of 1099 subjects, 561 were boys (51%), and 538 were girls (49%). Of the total subjects, 354 (32.2%) were children aged 3-years old, 441 (40.1%) were children aged 4-years, 276 (25.2%) were children aged 5-years, and 28 were children (2.5%) aged 6-years old. The result of SEM showed that the children's gross motor, fine motor and general motor ability was correlated positively with children's physical growth (P < 0.001), their standardized estimation of structural coefficient were 0.40, 0.36, 0.37 respectively. The result of multi-group SEM showed that there was no significant difference in the SEM between different gender and ages. The fine motor ability was positively correlated with child physical growth (P < 0.001). There was no inter-group difference in the structural model (each P > 0.05). However, there was difference in the structural model between overweight and normal group, of them, only the structural coefficient of gross motor and physical growth were different between the two groups (t = 1.697, P < 0.05).</p><p><b>CONCLUSION</b>There is a relationship between children's motor coordination and physical growth. The degree of association between the gross moter and physical growth in overweight children were different from the normal children. Further study on the factors influencing the children's physical growth is needed.</p>

Associations of human leukocyte antigen-A, B, DRB1 genes with leukemia patients in Anhui province of China / 中国实验血液学杂志

This study was aimed to investigate the relation of human leukocyte antigen-A, B, DRB1 genes with the susceptibility of population to leukemia in Anhui province of China. The HLA genotypes were analyzed by PCR-SSP in 140 patients with chronic myelocytic leukemia (CML), 84 patients with acute lymphoblastic leukemia (ALL), 90 patients with acute nonlymphocytic leukemia (ANLL) and 916 healthy unrelated donors of hematopoietic stem cell as normal control admitted to Anhui provincial hospital. The gene frequencies of HLA-A, B, DRB1 between patients and normal controls were compared, chi² test was used for statistical analysis. The results showed that as compared with normal controls, the gene frequencies of A2, A11, B58 and DR9 in CML patients all obviously increased, and gene frequency of DR7 decreased; the gene frequencies of All and B13 in ALL patients were significantly higher than that in normal controls; the gene frequencies of A24, B58 and DR9 in ANLL patients were significantly higher than that in normal controls. It is concluded that HLA-A2, A11, B58 and DR9 are predisposing genes of CML patients, DR7 is an antagonistic gene, HLA-A11 and B13 are predisposing genes of ALL patients, HLA-A24, B58 and DR9 are predisposing genes of ANLL patients.

Developmental characteristics and response to iron toxicity of root border cells in rice seedlings / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

To investigate the Fe2+ effects on root tips in rice plant, experiments were carried out using border cells in vitro. The border cells were pre-planted in aeroponic culture and detached from root tips. Most border cells have a long elliptical shape. The number and the viability of border cells in situ reached the maxima of 1600 and 97.5%, respectively, at 20-25 mm root length. This mortality was more pronounced at the first 1-12 h exposure to 250 mg/L Fe2+ than at the last 12-36 h. After 36 h, the cell viability exposed to 250 mg/L Fe2+ decreased to nought, whereas it was 46.5% at 0 mg/L Fe2+. Increased Fe2+ dosage stimulated the death of detached border cells from rice cultivars. After 4 h Fe2+ treatment, the cell viabilities were > or =80% at 0 and 50 mg/L Fe2+ treatment and were <62% at 150, 250 and 350 mg/L Fe2+ treatment; The viability of border cells decreased by 10% when the Fe2+ concentration increased by 100 mg/L. After 24 h Fe2+ treatment, the viabilities of border cells at all the Fe2+ levels were <65%; The viability of border cells decreased by 20% when the Fe2+ concentration increased by 100 mg/L. The decreased viabilities of border cells indicated that Fe2+ dosage and treatment time would cause deadly effect on the border cells. The increased cell death could protect the root tips from toxic harm. Therefore, it may protect root from the damage caused by harmful iron toxicity.

The Effects of DTBNP on Intracellular Ca2+ Signaling in Cultured Bovine Aortic Endothelial Cells

The mechanism underlying oxidant-induced intracellular Ca2+ ([Ca2+]i) increase was studied in cultured bovine aortic endothelial cells (BAECs) using fura-2 AM. In the presence of 2 mM extracellular Ca2+, the application of DTBNP (20microM), a membrane-permeable oxidant, caused an increase in [Ca2+]i, and DTT (2 mM) as a reductant completely reversed the effect of DTBNP. The [Ca2+]i increase induced by DTBNP was also observed in an extracellular Ca2+-free/2 mM EGTA solution, indicating the release of Ca2+ from intracellular store (s). After endoplasmic reticulum was depleted by an IP3-generating agonist, ATP (30microM) or an ER Ca2+ pump inhibitor, thapsigargin (1microM), DTBNP-stressed BAECs showed an increase of [Ca2+]i in Ca2+-free/2 mM EGTA solution. Ratio-differences before and after the application of DTBNP after pretreatment with ATP or thapsigargin were 0.42+/-0.15 and 0.49+/-0.07, respectively (n=7), which are significantly reduced, compared to the control value of 0.72+/-0.07 in a Ca2+-free/2 mM EGTA solution. After the protonophore CCCP (10microM) challenge to release mitochondrial Ca2+, the similar result was obtained. Ratio-difference before and after the application of DTBNP after pretreatment with CCCP was 0.46+/-0.09 (n=7). Simultaneous application of thapsigargin and CCCP completely abolished the DTBNP-induced [Ca2+]i increase. The above results together indicate that the increase of [Ca2+]i by DTBNP resulted from the release of Ca2+ from both endoplasmic reticulum and mitochondria.

Increase of Intracellular Ca2+ Concentration Induced by Lysophosphatidylcholine in Murine Aortic Endothelial Cells

Effects of oxidized low-density lipoprotein (ox-LDL), l-alpha-stearoyl-lysophosphatidylcholine (LPC), on intracellular Ca2+ concentration were examined in mouse endothelial cells by measuring intracellular Ca2+ concentration ([Ca2+]i) with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased [Ca2+]i under the condition of 1.5 mM [Ca2+]o but did not show any effect under the nominally Ca2+-free condition. Even after the store depletion with 30microM 2,5-di-tert- butylhydroquinone (BHQ) or 30microM ATP, LPC could still increase the [Ca2+]i under the condition of 1.5 mM [Ca2+]o. The time required to increase [Ca2+]i (about 1 minute) was longer than that for ATP-induced [Ca2+]i increase (10-30 seconds). LPC-induced [Ca2+]i increase was completely blocked by 1microM La3+. Transient receptor potential channel(trpc) 4 mRNA was detected with RT-PCR. From these results, we suggest that LPC increased [Ca2+]i via the increase of Ca2+ influx through the Ca2+ routes which exist in the plasma membrane.