RESUMEN
<p><b>OBJECTIVE</b>To compare the protective activity of liver injury induced by D-galactosamine (GalN) between Huangqin-Tang and their metabolites by human intestinal bacteria(HIB).</p><p><b>METHOD</b>The liver injuries in conventional and pseudo-germfree mice were induced by GalN. After oral administration of Huangqin-Tang and their metabolites mixtures by HIB, the serum transaminase (ALT and AST) activities were detected.</p><p><b>RESULT</b>In conventional mice, large and medium doses (20 and 10 g.kg-1) of Huangqin-Tang decoction significantly reduced the increase of serum ALT activity after 18 h GalN treatment. In pseudo-germfree mice, metabolites significantly reduced the ALT levels. However, Huangqing-Tang didn't affect the ALT levels in this kind of mice. To all of the animals, AST levels remained the same after oral Huangqin-tang or their metabolites.</p><p><b>CONCLUSION</b>The metabolism by intestinal bacteria plays a role in pharmacological effects of constituents of Chinese herbal medicine. The metabolites of the constituents by intestinal bacteria were the real active components in vivo.</p>
Asunto(s)
Animales , Masculino , Ratones , Administración Oral , Alanina Transaminasa , Sangre , Aspartato Aminotransferasas , Sangre , Bacterias , Metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas , Medicamentos Herbarios Chinos , Metabolismo , Farmacología , Galactosamina , Intestinos , Microbiología , Hepatopatías , Metabolismo , Plantas Medicinales , Química , Sustancias Protectoras , Metabolismo , FarmacologíaRESUMEN
<p><b>OBJECTIVE</b>To compare the antibacterial effects of Huangqin-Tang with its metabolites produced by intestinal flora.</p><p><b>METHOD</b>The antibacterial tests in vitro and in vivo were performed by agar dilution method and lethal protection of animal respectively.</p><p><b>RESULT</b>Huangqin-Tang and its metabolites had antibacterial action on bacteria in vitro, however the antibacterial activity of metabolites of Huangqin-Tang on Salmomella, Dysentery bacillus and Proteus in vitro was stronger than Huangqin-Tang. The metabolites of Huangqin-Tang had protective effect on the animals infected by Staphylococcus aureus and Escherichia coli respectively from death, but Huangqin-Tang had no lethal protection action.</p><p><b>CONCLUSION</b>The antibacterial effects of metabolites of Huangqin-Tang in vitro and in vivo are stronger than that of Huangqin-Tang, which shows that intestinal flora play a very important role in antibacterial effects of Huangqin-Tang.</p>