The dynamic change of galectin-1 in in mice with viral myocarditis / 临床儿科杂志

Objectives To investigate the dynamic change of galectin-1 in myocardium of mice with viral myocarditis (VMC). Methods A total of 58 male BALB/c mice were selected and randomly divided into CVB3 group (n=50) and control group (n=8). Mice in CVB3 group were infected with 0.10 ml 10-5/L CVB3 through intraperitoneal inoculation, and 8 mice were killed on day 7, 10, 14 and 28 respectively after inoculation. Mice in control group inoculated with 0.10 ml eagle reagent was killed on day 28. The myocardial pathological changes were observed using light microscope. In addition, expressions of serum galectin-1 were detected by ELISA and expressions of myocardial galectin-1 were detected by real-time quantitative-polymerase chain reaction (RQ-PCR). Results Compared with control group, the myocardial expression of galectin-1 mRNA in CVB3 group was obviously increased on day 7 and day 10 (all P<0.05);the serum concentration of galectin-1 in CVB3 group was obviously increased on day 7, day 10 and day 14 (all P<0.05). Conclusion The expressions of galectin-1 in myocardium of mice with viral myocarditis were in dynamic changes. Galectin-1 may play a role in the pathogenesis and development of viral myocarditis.

Characteristics of fructification and seed ripening of Panax stipuleanatus / 中国中药杂志

Characteristics of fructification and seed ripening of Panax stipuleanatus were studied in this paper with a view to better understand the species' biological characteristics and promote cultivation. Dynamic of fructification and types of fruits were observed in a period of 80 days. The seeds' biological characteristics were evaluated by observing embryo's vitality and changes in form in a period of 225 d. The findings and conclusions were as follows: A plant produces an average of (38.7 +/- 6.5) seeds; Seeds become fully mature 80 days after setting fruit, and fruits grow more rapidly in 0-40 days with an increase of 1 500.83% in fresh weight. Three types of fruits were found, that is mono-seed type, bi-seeds type and triple-seeds type, and the proportion is 9.53%, 58.66%, 1.81%, respectively. The seeds are (5.16-6. 52) mm in length and (3.12-4.95) mm in width. The weight of 1000 seeds is (0.648 +/- 0.014) g with a moisture content of (55.23 +/- 0.49)%. The after-ripening of embryo in Panax stipuleanatus is morphological form, embryos develop 75 days after the seeds' detaching from mother plant and become mature in 165-180 days with the ending of the process of after-ripening. The vitality of embryo reaches 70% , and the length of embryo is (3.217 +/- 0.406) mm and the width (0.872 +/- 0.165) mm. The seeds will germinate in 195 d.

Flowering biological characteristics of Panax stipuleanatus / 中国中药杂志

<p><b>OBJECTIVE</b>To reveal the flowering characteristics of Panax stipuleanatus and provide theoretical basis for distant hybridization between P. stipuleanatus and P. notoginseng.</p><p><b>METHOD</b>During the blossom of P. stipuleanatus, we observed and investigated blooming phenophase, growth dynamics of inflorescence, pollination and seed setting of its population and the flowering process and its period, the regularity of flowering and pollinating of the floret. Statistic analyses were carried out.</p><p><b>RESULT AND CONCLUSION</b>The population florescence of P. stipuleanatus was about 60 d. The average florets quality of umbel was 44-47. The average natural pollination rates were 73.32%-95.39%. The average seed setting rates was 35.65%-51.76%. The highest growth periods of inflorescence are from March 25 to April 4. The diameter, the height and the length of its inflorescence in 10 d increased 44.65%, 42.19% and 106.25%, respectively. The whole stereotype period was the May beginning. The time that the floret from petal opening to withering it generally needed 36-48 h, 60 percent of the floret finished auther pollinating during the same day and 40 percent until the next day. The flowering and pollinating peak periods were 14:00-15:00, the flowering numbers was 28.48% of the total flowering amount and the pollinating numbers were 38.63% of the total pollinating amount. The high temperature (20-30 degrees C) and the low humidity (RH < 60%) were beneficial to flowering and pollinating.</p>