Activation of intestinal mucosal TLR4/NF-κB pathway is associated with renal damage in mice with pseudo-sterile IgA nephropathy / 细胞与分子免疫学杂志

Objective To investigate the effect of intestinal mucosal Toll-like receptor 4/nuclear factor κB (TLR4/NF-κB) signaling pathway on renal damage in pseudo-sterile IgA nephropathy (IgAN) mice. Methods C57BL/6 mice were randomly divided into experimental group (pseudosterile mouse model group), control group (IgAN mouse model group), pseudosterile mouse blank group, and normal mouse blank group. Pseudosterile mice were established by intragastric administration of quadruple antibiotics once a day for 14 days. The pseudosterile IgAN mouse model was set up by combination of oral bovine serum albumin (BSA) administration and staphylococcal enterotoxin B (SEB) injection. The pathological changes of renal tissue were observed by immunofluorescence staining and PAS staining, and the intestinal mucosa barrier damage indicators lipopolysaccharide(LPS), soluble intercellular adhesion molecule 1(sICAM-1) and D-lactate(D-LAC) were analyzed by ELISA. Biochemical analysis was used to test 24 hour urine protein, serum creatinine and blood urea nitrogen. The mRNA and protein levels of Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and nuclear factor κB (NF-κB) were detected by reverse transcription PCR and Western blot analysis. Results The kidney damage of pseudosterile IgAN mice was more severe than that of IgAN mice, and the expressions of intestinal mucosal barrier damage markers (LPS, sICAM-1 and D-LAC) were significantly increased in pseudosterile IgAN mice. In addition, the expressions of TLR4, MyD88, and NF-κB level were all up-regulated in the intestinal tissues of IgAN pseudosterile mice. Conclusion Intestinal flora disturbance leads to intestinal mucosal barrier damage and induces activation of TLR4 signaling pathway to mediate renal injury in IgAN.

Effectiveness of controlled-release dinoprostone on odinopoeia in prolonged pregnancy / 中国生化药物杂志

Objective To investigate the effect of controlled-release dinoprostone on odinopoeia in prolonged pregnancy.Methods 94 cases of prolonged pregnancy women selected in First People's Hospital in Pinghu City from January 2012 to November 2014 were randomly divided into two groups, 47 cases in each group.The control group were given misoprostol to induce labour, while the observation group were given controlled-release dinoprostone to induce labour.The Bishop score, odinopoeia effect and adverse reactions were compared between two groups and expressions of prostaglandin E2(PGE2) and PGF2αin deciduas of observation group were compared between induction success group and induction failure group. Results The Bishop score in observation group was higher than that in control group 12h post-administration(P<0.05).The total efficiency of cervical ripening, the induction success rate in observation group were higher than those in control group(P<0.05).The adverse reactions and the incidence of complications between two group was not statistically significant.The PGE2 and PGF2αconcentrations in induction success group were higher than those in induction failure group(P<0.05).Conclusion The controlled-release dinoprostone could achieve similar security of misoprostol in odinopoeia, but has more significant induction effect.