Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Añadir filtros








Intervalo de año
1.
Artículo en Chino | WPRIM | ID: wpr-1038506

RESUMEN

Objective@#To explore and optimize the primary culture method of neonatal mouse hippocampal neurons in vitro.To construct a G-protein-coupled receptor kinase 2 ( GRK2) knockout HT22 cell line.@*Methods @#Hippocampal tissue of C57BL6 /J mice on day 1-2 was taken,digested with trypsin and pipetted to form a cell suspension,and supplement was added to Neurobasal-A medium to maintain cell growth. CRSIPR / Cas9 gene editing technique was used to construct HT22-GRK2 -/ - cell line,and the knockout efficiency of GRK2 was detected by immunofluorescence staining and Western blot. @*Results @#Primary hippocampal neurons of newborn mice were put into six-well plates with 3 × 107 /well using a serum-free culture method,which could get a high purity and good activity ; HT22-GRK2 -/ - cell line was constructed successfully.@*Conclusion@#The primary culture method of mouse hippocampal neurons was successfully established and optimized,and HT22-GRK2 -/ - cell line was successfully constructed by CRSIPR / Cas9 gene editing technique.

SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA