RESUMEN
Objective: For the first time, we used molecular signaling pathway enrichment analysis to determine possible involvement of miR-126 and IRS-1 in neurotrophin pathway
Materials and Methods: In this prospective study, validated and predicted targets [targetome] of miR-126 were collected following searching miRtarbase [http://mirtarbase.mbc.nctu.edu.tw/] and miRWalk 2.0 databases, respectively. Then, approximate expression of miR-126 targeting in Glioma tissue was examined using UniGene database [http://www.ncbi. nlm.nih.gov/unigene]. In silico molecular pathway enrichment analysis was carried out by DAVID 6.7 database [http://david. abcc.ncifcrf.gov/] to explore which signaling pathway is related to miR-126 targeting and how miR-126 attributes to glioma development
Results: MiR-126 exerts a variety of functions in cancer pathogenesis via suppression of expression of target gene including PI3K, KRAS, EGFL7, IRS-1 and VEGF. Our bioinformatic studies implementing DAVID database, showed the involvement of miR-126 target genes in several signaling pathways including cancer pathogenesis, neurotrophin functions, Glioma formation, insulin function, focal adhesion production, chemokine synthesis and secretion and regulation of the actin cytoskeleton
Conclusion: Taken together, we concluded that miR-126 enhances the formation of glioma cancer stem cell probably via down regulation of IRS-1 in neurotrophin signaling pathway