Bacteriological quality of drinking water of Sukkur City

To determine the bacteriological quality of drinking water of Sukkur city. A descriptive study was carried out at the Department of Microbiology, Diagnostic and Research Centre, Shah Abdul Latif University, Khairpur, Sindh from January to December 2007. A total of 120 samples were analyzed by membrane filter method. Drinking water samples from main storage, distribution line and consumer taps were analyzed. All 120 samples [100%] were found contaminated with total coliform bacteria and 98 [82%] samples were found to be contaminated with thermotolerant Escherichia coli. Chlorine [residual] was not found in any of the samples. Drinking water samples were heavily contaminated with coliform and faecal coliform organisms making it unsuitable for drinking. Pakistan population should be encouraged to use boiled water for drinking, till the time that safe drining water is made available to all

Enumeration of total and fecal coliform bacteria in drinking water of Khairpur Sindh

The number and range of total coliform and fecal coliform bacteria were analyzed in the drinking water of Khairpur city to determine the quality of the water supplied to the residents of the city. The bacteriological study was carried out for indicator organisms i.e total coliform and fecal coliform [E. coli] by membrane filtration methods. A total of 90 samples were collected from three sites, thirty samples each from main reservoir [water works], distribution line and consumer taps [households, hotels and schools]. All samples were found contaminated [100%] with total coliform [TC] as well as fecal colifom [FC] bacteria. The counts were higher than the maximum microbial contaminant level established by World Health Organization [WHO]. The higher densities of bacteria were found in consumer taps [4-4.30 log cfu/100 ml] per 100 ml, followed by distribution line [3.79-4.20] and main reservoir [3-3.94 log cfu] per 100 ml total and fecal coliform. The pH and residual chlorine of water samples were also determined. It was observed that pH was within the limits of WHO standard [6.5-8.5], the residual chlorine was not detected in any sample of drinking water

Hair bait technique a method for isolation of the keratinases producing micro-organisms from soil

This paper states the methods of keratin baiting for isolation of keratinases producing microbes. Hair Bait Technique [HBT] is a specific method for isolation of the keratinases producing microbes [fungi and bacteria] from soil. There are several industrial and domestic applications of these enzymes

Isolation, identification and occurrence of medically important keratinophilic fungi from soil of taluka Dokri, district Larkana, Sindh, Pakistan

Prevalence of keratinophilic fungi with some exceptions is reported everywhere in soil hitch may be more or less pathogenic to man causing dermal infections. This presentation is based on the total percentage of these keratinophilic fungi from various talukas of district Larkana. Forty-five samples of surface soil, 10 cm. and 20 cm, depth from various locations of taluka Dokri were collected and examined for the occurrence of medically important keratinophilic fungi. Our results revealed the highest percentage of Aspergillus jlavus followed by Aspergillus wentii, Microsporum nanum, Botlytis cinerea and Trichophyton equinum respectively. Their variation is based on the physical and chemical properties of soil. Laboratory investigation revealed large macular skin and mild hair infection in lab. Animals caused by Microsporum nanum and Trichophyton equinum as pathogenic fungi. General survey of the occurrence of dermatomycosis in taluka Dokri revealed 42% in old age [above 50 years] and 21% in children [up to 15 years]