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Artículo en Inglés | WPRIM | ID: wpr-820520

RESUMEN

OBJECTIVE@#To evaluate the antimicrobial efficacy of berberine, a plant alkaloid.@*METHODS@#Five multi-drug resistant (MDR) STEC/EPEC and five MDR ETEC isolates from yaks with haemorrhagic diarrhoea were selected for the study. Antibacterial activity of berberine was evaluated by broth dilution and disc diffusion methods. The binding kinetics of berberine to DNA and protein was also enumerated.@*RESULTS@#For both categories of enterovirulent Escherichia coli (E. coli) isolates, berberine displayed the antibacterial effect in a dose dependent manner. The MIC(50) of berberine chloride for STEC/EPEC isolates varied from 2.07 μM to 3.6 μM with a mean of (2.95 ± 0.33) μM where as for ETEC strains it varied from 1.75 to 1.96 μM with a mean of (1.87 ± 0.03) μM. Berberine bind more tightly with double helix DNA with Bmax and Kd of (24.68±2.62) and (357.8±57.8), respectively. Berberine reacted with protein in comparatively loose manner with Bmax and Kd of (18.9±3.83) and (286.2±113.6), respectively.@*CONCLUSIONS@#The results indicate clearly that berberine may serve as a good antibacterial against multi drug resistant E. coli.


Asunto(s)
Animales , Bovinos , Antibacterianos , Usos Terapéuticos , Berberina , Metabolismo , Usos Terapéuticos , Enfermedades de los Bovinos , Quimioterapia , ADN Bacteriano , Metabolismo , Diarrea , Quimioterapia , Farmacorresistencia Bacteriana Múltiple , Escherichia coli Enteropatógena , Escherichia coli Enterotoxigénica , Infecciones por Escherichia coli , Quimioterapia , Pruebas de Sensibilidad Microbiana , Unión Proteica , Escherichia coli Shiga-Toxigénica
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