Cell-assisted lipotransfer for breast augmentation: a report of 18 patients / 中华整形外科杂志

<p><b>OBJECTIVE</b>To evaluate the clinical effect of cell-assisted lipotransfer (CAL) for breast augmentation.</p><p><b>METHODS</b>18 patients accepted breast augmentation using CAL. 10 patients completed 6-month follow-up and were involved in the study. The adipose tissue was harvested from patients' thighs, flanks and lower abdomen with Lipokit. After standing, 250 ml fatty portion and 500 ml fluid portion of suction aspirates were processed according to the procedures reported in reference. Flow-cytometry was used to detect the percentage of adipose-derived stem cells(ADSCs) in distilled stromal vascular fraction (SVF). The differentiation function of cultured cells also was assessed. The breast volume and images were evaluated by using MRI before operation, 3 and 6 months after operation. The breast volume was marked as V0, V3 and V6 respectively. The resorption rate of transplanted adipose tissue for each breast was calculated and marked as R3 and R6.</p><p><b>RESULTS</b>Averagely, the percentage of ADSCs in freshly distilled SVF was 41.67%. The in-vitro cultured cell grew well and could differentiate into fat, bone and cartilage. Statistics showed that V0, V3 and V6 was (416.19 +/- 40.43) ml, (551.72 +/- 59.86) ml and (538.81 +/- 68.35) ml respectively. R3 and R6 was (51.20 +/- 11.96)% and (54.22 +/- 12.73)%. There was significant difference between V3 and V0 (P < 0.05), V6 and V0. However, no significant difference was showed between V3 and V6 or R3 and R6. In addition, no cyst or calcification was seen in all MRI images.</p><p><b>CONCLUSIONS</b>In process of breast augmentation using CAL, the distilled SVF contains 41.67% ADSCs which have adipogenic, osteogenic and chondrogenic function. Within 3-month post-operation, the breast volume decreases obviously but the volume sustains after that. Compared with the preoperative volume, the 6-month postoperative volume is significantly increased and the breasts' contour is improved greatly. This study indicates that CAL is a safe and effective way for breast augmentation.</p>

Effects of antisense oligonucleotides on the expression of focal adhesion kinase gene and collagen synthesis in the cultured human fibroblasts of hypertrophic scar / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study the role of focal adhesion kinase (FAK) in the pathogenesis of human hypertrophic scar.</p><p><b>METHODS</b>Human hypertrophic scar fibroblasts (HSFB) were isolated from human hypertrophic scar and cultured in vitro. The cells were then divided into 3 groups as AT group (phosphorothioate FAK ASODN was transfected into the HSFB by liposome), LPC group (liposome only), and LC group (control group, without liposome or ASODN). The FAKmRNA index of HSFB was assessed by polymerase chain reaction method (FQ-PCR). The collagen synthesis of HSFB was assessed by 3H-proline incorporation method.</p><p><b>RESULTS</b>The FAK mRNA index of HSFB in AT group 48 hours after transfection was significantly lower than that in LPC and LC groups (0.043 +/- 0.030, 0.124 +/- 0.070, 0.127 +/- 0.0195, P < 0.05). The 3H-proline incorporation rate in AT group was lower than that in LPC and LC groups (257.0 +/- 15.14, 962.2 +/- 300.5, 930.8 +/- 28.97, P < 0.01).</p><p><b>CONCLUSION</b>The expression of FAK gene and collagen synthesis of the cultured HSFB could be inhibited by FAK ASODN, indicating that FAK played a role in the development of excessive fibrosis of human hypertrophic scar.</p>