Correlation of Left Mandibular Third Molar Development and Chronological Age / 대한소아치과학회지

The aim of this study is to evaluate the development of the left mandibular third-molar development with modified Demirjian method and its relation to chronological age.A total of 1653 digital panoramic radiographs of healthy individuals aged between 7 and 23 years who visited Yonsei University Dental hospital were selected. The developmental status of the left mandibular third molars was assessed using dental maturity scoring proposed by Demirjian et al. The mean age of the first appearance of mineralization, complete crown formation, and complete root formation were around 9, 14, 21 years respectively. Statistically significant differences between males and females in the development stage of D and G were revealed that crown formation and root length completion were attained earlier in males than in females. There was significant positive relationship between age and third molar development in both sexes, and new formula was presented to estimate age of children and adolescents based on their developmental stages of third molars.In this study, the use of left mandibular third molar as a developmental marker is appropriate, and age estimation can be attained with dental maturity stage.

Accuracy of Automatic Cephalometric Analysis Programs on Lateral Cephalograms ofPreadolescent Children / 대한소아치과학회지

The aim of this study was to evaluate the accuracy of 3 different automatic landmark identification programs on lateral cephalgrams and the clinical acceptability in pediatric dentistry.Sixty digital cephalometric radiographs of 7 to 12 years old healthy children were randomly selected. Fourteen landmarks were chosen for assessment and the mean of 3 measurements of each landmark by a single examiner was defined as the baseline landmarks. The mean difference between an automatically identified landmark and the baseline landmark was measured for each landmark on each image.The total mean difference of 3 automatic programs compared to the baseline landmarks were 2.53 ± 1.63 mm. Errors among 3 programs were not significantly different for 12 of 14 landmarks except Orbitale and Gonion. The automatic landmark identification programs showed significant higher mean detection errors than the manual method. The programs couldn’t be used as the 1st tool to replace human examiners. But considering short consuming time, these results indicate that all 3 programs have sufficient validity to be used in pediatric dental clinic.

Effects of Nursing Professionalism and Job Stress on the Problem Solving Ability of Community Health Practitioners / 간호행정학회지

Purpose@#The purpose of this study was to examine the influences of nursing professionalism and job stress on problem solving ability of community health practitioners. @*Methods@#The participants were 167 community health practitioners. Data were collected from December 18 to 28, 2019. Self-report questionnaires were administered to collect data regarding general characteristics, degree of nursing professionalism, job stress, and problem solving ability. Data were analyzed using descriptive statistics, independent t-test, One-way ANOVA, Pearson correlation coefficients and multiple regression analysis. @*Results@#The factors influencing the degree of problem solving ability of the community health practitioners were professionalism of nursing (β=.29, p<.001), and interpersonal factors (β=-.25, p=.001). It was found that 15% could explain problem solving ability. @*Conclusion@#It is necessary to strengthen the professionalism of nursing and to decrease the interpersonal factors due to job stress for the community health practitioners. The results indicate a need to increase development of educational programs for capacity building of community health practitioners.

Factors Influencing the Infection Control Performance of Catheter-Associated Urinary Tract Infection of Nurses in Hospitals / 임상간호연구

PURPOSE@#The purpose of this study was to examine nurses' knowledge, health beliefs, and performance regarding the infection control of catheter-associated urinary tract infection (CAUTI) and to identify factors affecting their infection control performance of the CAUTI.@*METHODS@#The subjects were 166 nurses at three hospitals with less than 300 beds in urban areas.Data were collected using structured questionnaires about knowledge, health beliefs, and performance regarding the infection control of the CAUTI. Statistical analysis included t-test, ANOVA, Pearson's Correlation Coefficients, Multiple regression analysis.@*RESULTS@#The factors affecting the infection control performance of CAUTI were knowledge (β=.18, p=.010), perceived seriousness (β=.25, p=.001), perceived barriers (β=.41, p<.001), and cues to action (β=.15, p=.030), and these factors explained 28.7% of the variance for the infection control performance of the CAUTI.@*CONCLUSION@#In this study, higher levels of knowledge, perceived seriousness and, cues to action, and lower perceived barriers resulted in a corresponding higher performance in the infection control of CAUTI. Based on these findings, providing intervention programs enhancing the health beliefs of nurses is necessary to ensure their infection control performance of the CAUTI.

Self-efficacy, Depression and Health Promotion Behaviors of Nursing and Non-nursing Female College Students / 한국간호교육학회지

PURPOSE: The purpose of this study was to compare the self-efficacy, depression, and health promotion behaviors of nursing and non-nursing female college students, and to develop a program to improve the health promotion behaviors of female college students. METHODS: One hundred sixty-six females (84 nursing, 82 non-nursing) college students participated in the study. Data collection was conducted between November 20 and December 8, 2017. Data was analyzed using descriptive statistics, χ2 test, ANCOVA, t-test, ANOVA, Pearson's correlation coefficients and stepwise multiple regression analysis with the SPSS/WIN 22.0. RESULTS: The results of health promotion behaviors of the nursing college students were higher than non-nursing college students (F=7.14, p=.008). Both nursing and non-nursing college students showed a positive correlation between health promotion behaviors and self-efficacy, and there was a negative correlation between health promotion behaviors and depression. Self-efficacy was the most influential factor in health promotion behaviors for both nursing and non-nursing female college students. CONCLUSION: In order to improve the health promotion behaviors of female college students, it is necessary to educate individuals to help them improve their self-efficacy and be able to maintain healthy lifestyles by perceiving and managing their own health conditions.

A Study on Physiological Index, Anxiety and Depression by the Severity of Lower Urinary Tract Symptoms in Patients with Benign Prostatic Hyperplasia

PURPOSE: The purpose of this study was to investigate the physiological index, anxiety and depression by the severity of lower urinary tract symptoms in patients with benign prostatic hyperplasia. METHODS: This research was conducted from the 4th to the 27th of May in 2016 on112 patients with benign prostatic hyperplasia. The data were analyzed using a chi-square test, ANOVA, and Pearson Correlation Coefficients. RESULTS: The results demonstrated a difference depending on the dysuria period of each lower urinary tract symptom, marital status, occupation and perceived health state of the patient. The physiological index by lower urinary tract symptoms showed a difference in the maximum flow rate, amount of post-void residual urine and Prostate-Specific Antigen (PSA), and anxiety and depression factors also revealed a difference. Lower urinary tract symptoms showed a positive correlation to the amount of post voided residual urine and PSA, a negative correlation to the maximum flow rate and also indicated a positive correlation to depression. CONCLUSION: The results of the study belonging to the moderate and severe categories were high when the result was based on the categorization of subjects with benign prostatic hyperplasia with lower urinary tract symptoms. Considering that depression levels increase as the symptom intensifies and the anxiety score is also high with mild symptoms, psychological support intervention is needed when a pattern of benign prostatic hyperplasia appears.

Effects of Nordic Walking on Body Composition, Muscle Strength, and Lipid Profile in Elderly Women

PURPOSE: The purpose of this study was to investigate the effects of Nordic walking on body composition, muscle strength, and lipid profile in elderly women. METHOD: Sixty-seven women were assigned to the Nordic walking group (n = 21), the normal walking group (n = 21), and the control group (n = 25). Nordic walking and normal walking were performed three times a week for 12 weeks. Body weight, body mass index, total body water, skeletal muscle mass, percent body fat, grip strength, sit to stand, arm curl, total cholesterol, triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were measured before and after the program. A Chi-square test, one way analysis of variance, paired t test and repeated-measure two-factor analysis were used with the SAS program for data analysis. RESULTS: There was a significant difference in the weight (F = 8.07, p < .001), grip strength (F = 10.30, p < .001), sit to stand (F = 16.84, p < .001), arm curl (F = 41.16, p < .001), and total cholesterol (F = 5.14, p = .009) measurements between the groups. In addition, arm curl was significantly increased in the Nordic walking group compared to the normal walking group and the control group. CONCLUSION: The results indicate that Nordic walking was more effective than normal walking in improving upper extremity strength.

RNA Mapping of Mutant Myotonic Dystrophy Protein Kinase 3'-Untranslated Region Transcripts

Myotonic dystrophy type 1 (DM1), which is a dominantly inherited neurodegenerative disorder, results from a CTG trinucleotide repeat expansion in the 3'-untranslated region (3'-UTR) of the myotonic dystrophy protein kinase (DMPK) gene. Retention of mutant DMPK (mDMPK) transcripts in the nuclei of affected cells has been known to be the main cause of pathogenesis of the disease. Thus, reducing the RNA toxicity through elimination of the mutant RNA has been suggested as one therapeutic strategy against DM1. In this study, we suggested RNA replacement with a trans -splicing ribozyme as an alternate genetic therapeutic approach for amelioration of DM1. To this end, we identified the regions of mDMPK 3'-UTR RNA that were accessible to ribozymes by using an RNA mapping strategy based on a trans - splicing ribozyme library. We found that particularly accessible sites were present not only upstream but also downstream of the expanded repeat sequence. Repair or replacement of the mDMPK transcript with the specific ribozyme will be useful for DM1 treatment through reduction of toxic mutant transcripts and simultaneously restore wild-type DMPK or release nucleus-entrapped mDMPK transcripts to the cytoplasm.

Effect of Hand Massage on Fatigue, Sleep Satisfaction and Blood Pressure of the aged in a Long-term Care Facility / 성인간호학회지

PURPOSE: The purpose of this study was to examine the effects of hand massage on fatigue, sleep satisfaction and blood pressure of the aged in a long-term care facility. METHODS: The data were collected from June to August 2008. The subjects were randomly recruited from a long-term care facility in Junnam Providence and were randomly assigned to the experimental group (n = 22) or control group (n = 22). Hand massage was carried out every other day in the evening for two weeks. Massage was administered to each forearm and hand for 10 minutes. Descriptive statistics, x2-test and t-test were performed using SAS program. RESULTS: In the experimental group, the fatigue (p = .017) was significantly lower and the sleep satisfaction (p = .043) was significantly higher than for the control group. CONCLUSION: On the basis of the results of the study, hand massage can be recommended for the elderly with sleep disturbance to reduce fatigue.

Effects of Resistance Exercise Combined to Relax Therapy in Blood Pressure, Frequency of Exercise and Physical Fitness in elderly Women in Rural District / 성인간호학회지

PURPOSE: This study was object to the effects of a resistance exercise combined with relaxation therapy on blood pressure, frequency of exercise and physical fitness in elderly women. METHODS: Seventy three elderly women participated in an exercise program for 12 months. The exercise program consisted of stretching, massage and resistance training using thera-bands twice a week and 50 minutes in every sessions each week. Repeated measures ANOVA were performed using SAS. RESULTS: After the exercise program there was a significant difference in systolic blood pressure, frequency of exercise, body fat, sitting to standing, walking 2 minutes, sitting on a chair and reaching, and standing on one leg with eyes opened. After 6 months, there was a significant decrease in systolic blood pressure and significant increase in frequency of exercise, body fat, sitting to standing and standing on one leg with eyes opened. In 12 months, there was a significant increase in body fat, 2 minutes walking, and sitting in a chair and reaching. CONCLUSION: Resistance exercise combined with muscle relaxation therapy would have a positive effect on elderly women. We found that it was helpful for the elderly women in terms of physical fitness.

Effects of Diabetes Education and Telephone Counseling on Depression in Patients with Diabetes / 성인간호학회지

PURPOSE: This study was conducted to identify the effects of diabetes education and telephone counseling on depression in diabetic patients. METHODS: 49 diabetic patients(24 in the control group and 25 in the experimental group) completed diabetes education and telephone counseling. Data were analyzed using chi-square test, paired t-test and unpaired t-tests. RESULTS: There were no significant differences in depression between the experimental and control groups. But, Depression scores were much decreased in the experimental group. CONCLUSION: Depression decreased following the diabetes education and telephone counseling. These findings indicate that diabetes education and telephone counseling could be one of the way to decrease depression.

In Vivo Target RNA Specificity of Trans-Splicing Phenomena by the Group I Intron

The Tetrahymena group I intron has been shown to employ a trans-splicing reaction and has been modified to specifically target and replace human telomerase reverse transcriptase (hTERT) RNA with a suicide gene transcript, resulting in the induction of selective cytotoxicity in cancer cells that express the target RNA, in animal models as well as in cell cultures. In this study, we evaluated the target RNA specificity of trans -splicing phenomena by the group I intron in mice that were intraperitoneally inoculated with hTERT-expressing human cancer cells to validate the anti-cancer therapeutic applicability of the group I intron. To this end, an adenoviral vector that encoded for the hTERT-targeting group I intron was constructed and systemically injected into the animal. 5'-end RACE-PCR and sequencing analyses of the trans-spliced cDNA clones revealed that all of the analyzed products in the tumor tissue of the virus-infected mice resulted from reactions that were generated only with the targeted hTERT RNA. This study implies the in vivo target specificity of the trans - splicing group I intron and hence suggests that RNA replacement via a trans -splicing reaction by the group I intron is a potent anti-cancer genetic approach.

Difference in Knowledge and Learning Needs of the Coronary Artery Disease according to the General Characteristics of the Patients with Type 2 Diabetes Mellitus / 기본간호학회지

PURPOSE: The purpose of this study was to assess the difference in knowledge and learning needs of the coronary artery disease according to the general characteristics of the patients with type 2 diabetes mellitus. METHOD: The participants were 188 patients who had diabetes mellitus. Data were obtained between January and April 2006 by asking the knowledge and learning needs of the coronary artery disease. Data were analyzed using SAS program. RESULTS: Participants' knowledge level was high in the cause and prevention, but the level was low in the symptom and occurrence of pain. Learning need for "Influence of smoking on heart disease" and "The structure and functions of the heart" was great, but learning need for "Complete diagnosis" and "Management of pain and pressure on the sternum" was little. No significant differences were found in the knowledge level of the coronary artery disease according to the general characteristics. Learning needs were greater in participants under 60 years of age (p=0.011) and in those with low education level (p=0.049). There was a significant correlation between knowledge and learning needs of the coronary artery disease (p=0.003). CONCLUSION: In planing the education programs, the general characteristics of the patients should be considered.

Knowledge and Learning Needs Related to Coronary Artery Disease in Diabetic Patient by Glycemic Control / 성인간호학회지

PURPOSE: The purpose of this study was to investigate the knowledge and learning needs on coronary artery disease in diabetic patients by glycemic control. METHODS: The subjects consisted of 188 patients at the hospital who had diabetes mellitus. Data was obtained using a knowledge and learning needs questionnaire from January to April 2006. RESULTS: Treatment method, the levels of fasting blood glucose(FBG), and 2-hour postprandial blood glucose (PP2hr) showed meaningful differences between normo-glycemic group(HbA1c or =7%). The levels of knowledge on coronary artery disease by glycemic control tend to show higher in normo-glycemic group. Etiology and prevention of coronary artery disease were significantly higher in normo- glycemic group than in hyper-glycemic group. The levels of learning needs on coronary artery disease by glycemic control tend to show higher in normo-glycemic group. The learning needs on items of diet control were higher in normo-glycemic group than in hyper-glycemic group. CONCLUSION: On the base of these results, we should focus on the coronary artery disease education for hyper-glycemic group. Also individual coronary artery disease educational program should be developed for the patients with different level of knowledge and learning needs.

Gene expression responses in vivo by human telomerase reverse transcriptase (hTERT)-targeting trans-splicing ribozyme

A trans-splicing ribozyme which can specifically reprogram human telomerase reverse transcriptase (hTERT) RNA was previously suggested as a useful agent for tumor-targeted gene therapy. In this study, we evaluated in vivo function of the hTERT-targeting trans-splicing ribozymes by employing the molecular analysis of expression level of genes affected by the ribozyme delivery into peritoneal carcinomatosis mice model. To this effect, we constructed adenoviral vector encoding the specific ribozyme. Noticeably, more than four-fold reduction in the level of hTERT RNA was observed in tumor nodules by the systemic infection of the ribozyme-encoding virus. Such hTERT RNA knockdown in vivo induced changes in the global gene expression profile, including the suppression of specific genes associated with anti-apoptosis including bcl2, and genes for angiogenesis and metastasis. In addition, specific trans-splicing reaction with the targeted hTERT RNA took place in the tumors established as peritoneal carcinomatosis in mice by systemic delivery of the ribozyme. In conclusion, this study demonstrates that an hTERT-specific RNA replacement approach using trans-splicing ribozyme represents a potential modality to treat cancer.

An RNA Mapping Strategy to Identify Ribozyme-Accessible Sites on the Catalytic Subunit of Mouse Telomerase

Telomerase reverse transcriptase (TERT) is an enzymatic ribonucleoprotein that prolongs the replicative life span of cells by maintaining protective structures at the ends of eukaryotic chromosomes. Telomerase activity is highly up-regulated in 85-90% of human cancers, and is predominately regulated by hTERT expression. In contrast, most normal somatic tissues in humans express low or undetectable levels of telomerase activity. This expression profile identifies TERT as a potential anticancer target. By using an RNA mapping strategy based on a trans-splicing ribozyme library, we identified the regions of mouse TERT (mTERT) RNA that were accessible to ribozymes. We found that particularly accessible sites were present downstream of the AUG start codon. This mTERTspecific ribozyme will be useful for validation of the RNA replacement as cancer gene therapy approach in mouse model with syngeneic tumors.

A Study of Depression, Anxiety, Stress Response and Self-care by Gender in Diabetic Patients / 재활간호학회지

PURPOSE: The purpose of this study was to investigate the depression, anxiety, stress response and self-care, to analyze the correlation among depression, anxiety, stress response and self-care by gender, and to determine factors associated with self-care in diabetic patients. METHOD: The subjects of this study were 103 participants with diabetes mellitus. Data were analyzed by chi-square test, t-test, Pearson correlation coefficient and multiple regression analysis by using SAS program. RESULT: Items for self-care evaluation by gender were significant differences in hospital visit, hypoglycemia preparation, proper hygiene, taking a rest, foot injury check, drinking, and smoking. The female patients are more likely to have higher self-care score than the male patients. However, there were no differences in depression, anxiety and stress response by gender. In male patients, there were positive correlations between the degree of depression and stress response, the degree of anxiety and stress response. In female patients, there were positive correlations between the degree of depression and stress response, the degree of anxiety and stress response. In multiple regression analysis, gender and experienced admission is associated with self-care. CONCLUSION: We should consider integrated approaches for psychological problems in the management of diabetic patients.

The Effect of Methylene Blue on Nitric Oxide Production in a Rat Model of Acute Liver Injury Induced by Paraquat

PURPOSE: This study was to investigate if methylene blue (MB) inhibited lipid peroxidation, nitric oxide (NO) generation, and gene expression of iNOS in acute liver injury induced by paraquat and if the inhibitory effect of MB was dose dependent. METHODS: Female Sprague-Dawley rats were divided into four groups: the control group, the group treated only with paraquat, and the groups treated with paraquat and low or high dose of MB (2 mg/kg, 20 mg/kg). RESULTS: The liver histology, the malondialdehyde (MDA) level, and the NO concentration in blood and the expression of iNOS mRNA in liver tissue were measured. The liver histology showed decreased inflammatory responses in the MB-treated groups compared to the group treated with paraquat only. The amount of liver MDA decreased in the MB-treated groups compared to the paraquat-only-treated group. There was a significant decrease in the MDA level in the high-dose MB-treated group. The plasma NO concentration decreased more in the MB-treated groups (low and high) than in the paraquat-only-treated group. There was a significant decrease in the plasma NO concentration at 6 h in the high-dose MB-treated group. Gene expression of iNOS mRNA in the liver tissue was slightly lower in the MBtreated group than in the paraquat-only-treated group. CONCLUSION: In this study, MB had an the antioxidant effect and an inhibitory effect on the plasma NO concentration and the expression of iNOS mRNA in the liver. The inhibitory effect of MB was dose dependent.

Regulation of Sodium-iodine Symporter Expression by Retinoic Acid in Thyroid Cancer Cell Lines / 대한내분비외과학회지

PURPOSE: Response to radioiodine therapy for thyroid cancer is related to the loss of sodium-iodine symporter protein caused by dedifferentiation of thyroid cancer cells. So we aimed to study mRNA expression of CD97, dedifferentiation marker, and sodium-iodine symporter after retinoic acid treatment according to retinoids receptor status. METHODS: Thyroid cancer cell lines; ARO, FRO, NPA, TPO, and FTC133 were prepared. 5µM of all trans retinoic acid were administered to each cell lines and then expression of m RNA for retinoids receptors (RARα, RARβ, RARγ, RXRα, RXRβ, RXRγ), CD97, and Sodium-Iodine symporter by RT-PCR. RESULTS: RARs and RXRs were differently expressed in each cell line. After retinoic acid treatment, relative density of retinoic acid receptor mRNA were increased by time dependently in each cell line except TPO cell line. Expression of CD97 also was decreased in every cell lines (P<0.001). Retinoic acid increased expression of sodium-iodine symporter only in FTC133 cell line (P<0.001), and TSH or forskolin did not enhance NIS expression by retinoic acid. RARβ and RXRγ were expressed only in FTC 133cell line before treatment. Induction of sodium-iodine symporter by retinoids disappeared after RARβ specific antagonist LE135 or pan RXR antagonist PA452 administration. CONCLUSION: Retinoic acid reduced expression of CD97 in five thyroid cancer cell lines. However, retinoic acid could restore sodium-iodine symporter expression in only FTC 133 cell line specifically containing RARβ and RXRγ. Restoration of sodium-iodine symporter expression by retinoic acid is related to RARβ and RXRγ expression.

Disease-Related Stress Experienced by Women with Mastectomy: Q Methodology / 여성건강간호학회지

PURPOSE: To identify the type of disease-related stress experienced by women who received mastectomy for breast cancer using Q methodology. METHOD: Q sample included 30 statements obtained from literature and interviews with women with mastectomy. P sample consisted of 22 patients with mastectomy. The data were collected from November to December 2002 and analyzed using QUANL program. RESULT: Four types of disease-related stress experienced by women with mastectomy were found. Type 1 was manifested for a short duration following surgery and characterized by lowered self-esteem, feelings of emptiness and depression because of impaired body image. Type 2 was characterized by declined physical strength, resulting in frequent fatigue in the daily life and less intimate relationship with spouse. Type 3 was manifested by perfectionists with strong sense of self-pride who received or considered breast reconstruction surgery. Type 4 was reported by those who had long period of post-surgery. This type reported a strong sense of discomfort for wearing an artificial breast but didn't show any intention of trying breast reconstruction surgery. CONCLUSION: Women with mastectomy were found to experience different type of disease-related distress. Therefore, nurses should assess the type of stress the patient experiences following a mastectomy to provide appropriate nursing care.