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Objective To investigate serum levels of lipoprotein-associated phospholipase A2 (Lp-PLA2),high-sensitivity C-reactive protein (hs-CRP),complement component C1q (C1q) and homocysteine(HCY) in patients with Alzheimer's disease(AD),in order to provide a basis for establishing laboratory markers in AD patients.Methods One hundred AD patients and one hundred healthy controls from Beijing Hospital were selected.Serum levels of Lp-PLA2,C1q,hs-CRP and HCY were determined using a biochemistry analyzer.Serum levels of amyloid β-protein 40(Aβ40)and Aβ42 were measured using enzyme-linked immunosorbent assays.Results Serum levels of Aβ40,Aβ42,Lp-PLA2,hs-CRP,C1 q and HCY were higher in AD patients than in the control group[(66.0±14.0) pmol/L vs.(7.1±8.2) pmol/L,(7.2±1.4) pmol/L vs.(1.9±1.7) pmol/L,(510.6±140.1)U/L vs.(419.0±91.8) U/L,(2.8±3.4) mg/L vs.(1.2±0.7) mg/L,(218.0±58.4) mg/L vs.(194.8 ± 27.7) mg/L and (18.8 ± 9.3) μmol/L vs.(14.9 ± 5.2) μmol/L,all P ＜ 0.01],and the differences were greater in female subjects than in male subjectss.Conclusions High serum levels of Lp-PLA2,C1q,hs-CRP and HCY may be associated with Alzheimer's disease,while the exact relationships need to be further investigated.
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Objective To investigate serum vitamin D levels in middle-aged and elderly patients with diabetes mellitus (DM) only,hypertension only,dyslipidemia only,or a combination of these conditions.Methods We consecutively recruited patients aged 45 years or over with DM only (n=100),hypertension only (n=100),dyslipidemia only (n=100),and a combination of these three conditions (n=100) from Beijing Hospital and further included 100 healthy individuals aged 45 or over undergoing routine physical examinations to serve as the control group.Fasting venous blood was collected and analyzed with an automated biochemical analyzer for glucose (GLU),total cholesterol (TC),triglycerides (TG),high-density lipoprotein cholesterol (HDL-C),and low-density lipoprotein cholesterol (LDL-C);Vitamin D,in the form of 25 hydroxycholecalciferol (25[OH]D),was measured using an electrochemiluminescence (ECLIA) method;HbA1c was detected using cation exchange high performance liquid chromatography.Results There were significant differences in serum vitamin D levels between the four noncontrol groups[DM:(16.9±7.3) μg/L,hypertension:(16.4±7.5) μg/L,dyslipidemia:(15.3±6.9) μg/L,and the combination group (15.2±7.1) μg/L]and the control group [(21.0±7.5) μg/L](all P＜0.01),but no significant difference was found in vitamin D levels among the four noncontrol groups (all P ＞0.05).When subjects were classified as vitamin D sufficient (≥ 30.0 μg/L),insufficient (20.0-29.9μg/L),deficient (10.0-19.9 μg/L) and severely deficient (＜10.0 μg/L),the proportions of severely deficient and deficient subjects in the four diseased groups were higher than in the control group,and the highest proportion was found in the combination group.Compared with the four diseased groups,the control group showed the highest proportion of subjects with insufficient vitamin D.The control group had the highest proportion with sufficient vitamin D,whereas the combination group had the lowest.Conclusions Serum vitamin D levels in patients with DM,hypertension or dyslipidemia,or a combination of these conditions are significantly lower than in normal individuals.Attention should be paid to vitamin D levels and their alterations in these patients.