Study on variation of actinoside C in leaves of Actinidia kolomikta with different growth periods by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To determine actinoside C in the leaves of Actinidia kolomikta with different growth periods.</p><p><b>METHOD</b>The separation was performed at 25 degrees C on ZORBAX Extend C18 column (4.6 mm x 250 mm, 5 microm), using amixture of methanol and water (51:49) as a mobile phase. The flow rate was 1.2 mL x min(-1), and the wavelength for measurement was 267 nm.</p><p><b>RESULT</b>The results showed that the contents of actinoside C in the leaves of A. kolomikta were variety in different growth periods. Actinoside C could reach its highest content in the middle ten days of June, then the content would decrease in the middle ten days of July slightly, it could reach their lowest content in the middle ten days of August.</p><p><b>CONCLUSION</b>The optimal collective date for A. kolomikta are in the middle ten days of June.</p>

Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To determine 20(S)-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium.</p><p><b>METHOD</b>The separation was performed on ZORBAX EXEND C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water (85:15) as mobile phase with the rate of 1.2 mL x min(-1) at 25 degrees C, the wavelength for measurement was 203 nm.</p><p><b>RESULT</b>The calibration curve was linear in the range of 0.5-25 microg for 20(S)-ginsengnoside Rh2(r = 0.9999, n = 7). The average recovery was 99.7% (RSD= 1.0%).</p><p><b>CONCLUSION</b>This method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20(S)-ginsengnoside Rh2 is high by this hydrolysis method.</p>