RESUMEN
This study was aimed to establish the fingerprints of Qian Solidaginis Herba by HPLC. The fingerprints of Qian Solidaginis Herba were built by using Diamonsil C18 (200 mm í 4.6 mm, 5 μm) column and acetonitrile -0.2%H3PO4 aqueous solution in gradient as mobile phase. The flow rate was 1.0 mL·min-1. Detecting wavelength was set at 282 nm. The total 10 batches of Qian Solidaginis Herba samples and their adulterants were analyzed. The results showed that 13 peaks were identified as the fingerprints of Qian Solidaginis Herba. Under chromatographic conditions mentioned above. It was concluded that the HPLC fingerprints method was found to have satisfactory accuracy, sta-bility and reproducibility, which was a potential method for the quality control of Qian Solidaginis Herba.
RESUMEN
This study was aimed to establish the quality and quantity analysis methods of tectoridin contented in the Iris tectorum Maxim. Thin layer chromatography (TLC) method was used to give quality analysis on tectoridin in I. tectorum. And the HPLC method was used to give quantity analysis on tectoridin in I. tectorum. The Diamonsil C18 (200 mm í 4.6 mm, 5 μm) column was used as analytical column. The acetonitrile-0.2% phosphoric acid (20:80) was used as mobile phase at the flow rate of 1.0 mL·min-1. The detection wavelength was 265 nm and the column temperature was 25℃. The results showed that the quality analysis of I. Tectorum had specific identification with-out the interference of other ingredients. The amount of inlet tectoridin had a good linearity with the response val-ue of peak area in the range of 0.12~2.22 μg. The average recovery rate was 100.96%. It was concluded that this method was simple and reproducible, which can be used in the quality control of I. tectorum.
RESUMEN
This study was aimed to establish an HPLC method for the determination of chlorogenic acid, rutin and kaempferide in Solidago decurrens Lour. A Diamonsil C18 column (4.6 mm í 200 mm, 5 μm) was adopted with the mobile phase of acetonitrile-0.2% phosphoric acid. The flow rate was 1.0 mL·min-1. The column temperature was at 25℃. The wavelength of detection was set at 282 nm. The results showed that the calibration curve was linear over the range of 63.2~442.4 μg for chlorogenic acid (r = 0.999 3), 8.1~56.8 μg for rutin (r = 0.999 4) and 10.8~75.7μg for kaempferide (r = 0.999 8). The average recovery of chlorogenic acid was 98.6% (RSD = 1.4%), that of rutin was 99.2% (RSD = 0.8%) and that of kaempferide was 100.3% (RSD = 1.0%). It was concluded that the method was simple, economical and accurate with good reproducibility.
RESUMEN
Objective To research the TLC identification of geniposide in Lian-pu-yin.Methods Gardenia geniposide in Lian-pu-yin of 10 different samples were identified by TLC.Results Geniposide could be detected by TLC.Conclusion This method was simple and accurate.