Effect of radical resection combined with antiviral therapy on hepatitis B virus infection / 中国基层医药

Objective@#To study the effect of radical resection combined with antiviral therapy on hepatitis B virus infection.@*Methods@#From January 2012 to January 2016, 46 patients with hepatocellular carcinoma complicated with hepatitis B virus infection in the First People's Hospital of Jiashan County were enrolled in this study.All the subjects included in this study had complete clinical data and were willing to cooperate with this study.According to the random number table method, the patients were divided into two groups, with 23 cases in each group.The two groups were all treated by radical resection, the control group was not given anti-viral treatment after operation, the study group was given anti-virus combination therapy after surgery.The patients were followed up for more than 1 year.The levels of liver function[alanine aminotransferase (ALT), serum albumin (ALB), prealbumin (PA)]and hepatitis B virus (HBV-DNA) were measured before and after operation in both two groups.The incidence rate of postoperative complications, length of hospital stay, medical expenses and recurrence rate were analyzed.@*Results@#There were no statistically significant differences in ALT, ALB, PA and HBV DNA between the two groups before operation (all P>0.05). There were statistically significant differences between the two groups after operation[ALT: (97.74±33.27)U/L vs.(142.98±53.41)U/L, t=3.447; ALB: (34.57±5.01)g/L vs.(38.44±4.32)g/L, t=2.805; PA: (192.14±53.24)mg/L vs.(159.85±53.96)mg/L, t=2.042; HBV-DNA: (4.25±1.04)logcopes/mL vs.(6.22±1.18)logcopes/mL, t=6.006, all P<0.05]. The incidence rate of postoperative complications, hospitalization time, medical expense of the study group were significantly lower than those of the control group[complications: 8.70% vs.43.45%, χ2=7.215; hospitalization time: (17.43±4.53)d vs.(20.94±5.05)d, t=2.481; medical expenses: (3.28±1.24) million yuan vs.(4.68±1.17) million yuan, t=3.938, all P<0.05]. After follow-up for 1 year, both two groups had recurrence, the difference was not statistically significant between the two groups(P>0.05).@*Conclusion@#Hepatocellular carcinoma with hepatitis B virus infection in patients with radical resection and anti-virus combination therapy, can better improve the patients' liver function and viral indicators, shorten the hospital stay, reduce medical costs, and can reduce the incidence of complications, with high safety.

Genetic and phenotypic analysis of a rare case with homozygous Chinese Gγ (Aγδβ)-thal deletion / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To analyze the genotype of a patient suspected for thalassemia through a series of experiments.</p><p><b>METHODS</b>Conventional methods for detecting common thalassemia mutations was used in conjunction with multiplex ligation-dependent probe amplification (MLPA) in order to determine the genotype of the patient. Corresponding primers were designed for developing a Gap-PCR system for detecting rare type mutations.</p><p><b>RESULTS</b>The patient was identified as a homozygote for Chinese Gγ(Aγδβ)-thal deletion, with clinical manifestations tending to be intermediate or severe based on the hematological characteristics. A Gap-PCR system has been developed for detecting the above mutation with accuracy and rapidity.</p><p><b>CONCLUSION</b>The Chinese Gγ(Aγδβ)-thal is prevalent in southern China, and caution should be taken to avoid misdiagnosis. The Gap-PCR system for detecting Chinese Gγ(Aγδβ)-thal is suitable for extended applications for its simplicity and rapidity.</p>

Extraction and isolation technology of polysaccharide from mulberry leaves / 中草药

ZHANG Lin-hua, GAO Rui-chang, XU Ming-li (School of Chemical Engineering, Tianjin University, Tianjin 300072, China)