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Background: Expression of microRNA⁃320 (miR⁃320) is down regulated in acute pancreatitis, and the mechanism of its effect on acute pancreatitis is still unclear. Aims: To investigate the effect of miR⁃320 on intestinal injury in rats with acute pancreatitis and its mechanism. Methods: Rats were randomly divided into sham operation group, model group, miR⁃ 320 agonist group (agomir miR ⁃ 320 group), miR ⁃ 320 agonist control group (agomir NC group), JAK2 inhibitor group (AG490 group), and NF⁃κB pathway inhibitor group (PDTC group). The rat model of acute pancreatitis was established by retrograde injection of 5% sodium taurocholate to the bile duct. The automatic biochemical analyzer was used to detect serum levels of amylase and lipase; ELISA assay was used to detect serum levels of TNF⁃α and IL⁃1β; HE staining was used to observe the pathological changes of rat pancreas and ileum; TUNEL staining was used to observe cell apoptosis in rat ileum; real⁃time fluorescent quantitative PCR (RT⁃qPCR) was used to detect the expression of miR⁃320 in ileum tissue; Western blotting method was used to detect the expressions of JAK2/STAT3 and NF⁃κB signaling pathway related proteins in ileum. Results: Compared with sham operation group, the pancreas and ileum were severely injured in model group, and the pathological score and ileum cell apoptosis were significantly increased (P<0.05), serum levels of amylase, lipase, TNF⁃ α, and IL⁃1β were significantly increased (P<0.05), the expression of miR⁃320 in ileum tissue was significantly decreased (P<0.05), the ratios of p⁃JAK2/JAK2, p⁃STAT3/STAT3, p⁃p65/p65, and p⁃IκBα/IκBα in ileum tissue were significantly increased (P<0.05). Compared with model group, the pathological damages of pancreas and ileum in agomir miR ⁃ 320 group, AG490 group and PDTC group were reduced, and the pathological score and ileum cell apoptosis were significantly decreased (P<0.05), serum levels of amylase, lipase, TNF ⁃ α, and IL ⁃ 1β were significantly decreased (P<0.05), the expression of miR⁃320 in ileum tissue was significantly increased (P<0.05), the ratios of p⁃JAK2/JAK2, p⁃STAT3/STAT3, p⁃ p65/p65, and p⁃IκBα/IκBα in ileum tissue were significantly decreased (P<0.05). Conclusions: MiR⁃320 can improve the intestinal injury in rats with acute pancreatitis by inhibiting the activation of JAK2/STAT3 and NF⁃κB signaling pathways.
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Objective:To explore the effect of intensive nursing mode on the nursing of colostomy complications of patients with rectal cancer after MILES and its influence on psychological status and quality of life.Methods:62 patients with colostomy after Miles operation were selected as the observation group, and 62 patients with Miles operation after Miles operation between February 2015 and January 2017 were selected as the control group. Comparisons of stoma complications within 3 months after operation were compared between the two groups. Changes of psychological status (fear, depression, anxiety, sensitivity and obsessive-compulsive symptoms), quality of life (physical, cognitive, emotional, role and social function) were compared before and after intervention.Results:The total incidence of complications in the observation group was 11.29% (7/62), which was lower than that in the control group (37.10% (23 / 62). The difference was statistically significant ( χ2 value was 11.261). After 3 months of operation, the scores of phobia, depression, anxiety, sensitivity and obsessive-compulsive symptoms decreased, and the scores of body, cognition, emotion, role and social function increased ( P < 0.05). After 3 months of operation, the scores of phobia, depression, anxiety, sensitivity and the scores of obsessive-compulsive symptoms were (2.51 ± 0.49), (2.33 ± 0.57), (2.33 ± 0.57), (2.07 ± 0.54), (2.66 ± 0.53) and the scores of body, cognition, emotion, role and social function was(16.05 ± 5.45), (13.54 ± 4.13), (14.39 ± 4.26), (15.51 ± 4.73), (16.90 ± 5.03) respectively, which were significantly higher than those of the control group (1.93) ± 0.67), (1.47 ± 0.45), (1.83 ± 0.55), (1.44 ± 0.42), (1.82 ± 0.63), and (9.23 ± 3.25), (8.21 ± 2.44), (9.08 ± 3.13), (6.89 ± 2.25), (7.35 ± 2.17). The difference between the two groups was statistically significant ( t values were 4.263-13.730, P < 0.01). Conclusion:Intensive nursing intervention can significantly reduce the complications of colostomy patients after Miles operation, improve the bad psychological and emotional state, improve the quality of life, and the clinical nursing effect is satisfactory.