RESUMEN
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly spread around the world during 2020, but the precise time in which the virus began to spread locally is difficult to trace for most countries. Here, we estimate the probable onset date of the community spread of SARS-CoV-2 for heavily affected countries from Western Europe and the Americas on the basis of the cumulative number of deaths reported during the early stage of the epidemic. Our results support that SARS-CoV-2 probably started to spread locally in all western countries analysed between mid-January and mid-February 2020, thus long before community transmission was officially recognised and control measures were implemented.
Asunto(s)
Humanos , Neumonía Viral/epidemiología , Infecciones por Coronavirus/epidemiología , Infecciones Comunitarias Adquiridas/epidemiología , Neumonía Viral/transmisión , Américas/epidemiología , Infecciones por Coronavirus/transmisión , Infecciones Comunitarias Adquiridas/transmisión , Infecciones Comunitarias Adquiridas/virología , Europa (Continente)/epidemiología , Pandemias , Betacoronavirus , SARS-CoV-2 , COVID-19RESUMEN
Human bocaviruses (HBoV) are mainly associated with respiratory and gastroenteric infections. These viruses belong to the family Parvoviridae, genus Bocaparvovirus and are classified in four subtypes (HBoV1-4). Recombination and point mutation have been described as basis of parvovirus evolution. In this study three viral sequences were obtained from positives HBoV sewage samples collected in two Uruguayan cities and were characterised by different methods as recombinant strains. This recombination event was localised in the 5' end of VP1 gene and the parental strains belonged to subtypes 3 and 4. These three Uruguayan strains are identical at the nucleotide sequences in the analysed genome region of the virus. As far as we known, this study represents the first detection of HBoV recombinants strains in the Americas.
Asunto(s)
Humanos , Genoma Viral , Infecciones por Parvoviridae/virología , Bocavirus Humano/genética , Filogenia , Uruguay , Secuencia de Bases , Bocavirus Humano/aislamiento & purificación , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
A recent study showed that infectivity of Zika virus (ZIKV) Asian genotype was enhanced by an alanine-to-valine amino acid substitution at residue 188 of the NS1 protein, but the precise time and location of origin of this mutation were not formally estimated. Here, we applied a Bayesian coalescent-based framework to estimate the age and location of the ancestral viral strain carrying the A188V substitution. Our results support that the ancestral ZIKV strain carrying the A188V substitution arose in Southeastern Asia at the early 2000s and circulated in that region for some time (5-10 years) before being disseminated to Southern Pacific islands and the Americas.