RESUMEN
Objective: recent studies have reported dysregulated expression of matrix metalloproteinases [MMPs], especially MMP-2, MMP-9, tissue inhibitor of metalloproteinase-1, -2 [TIMP-1, TIMP-2], and extracellular matrix metalloproteinase inducer [EMMPRIN/CD147] in activated macrophages of patients with inflammatory diseases. Therefore, MMP-2, MMP-9, and their regulators may represent a new target for treatment of inflammatory diseases. Probiotics, which are comprised of lactic acid bacteria, have the potential to modulate inflammatory responses. In this experimental study, we investigated the anti-inflammatory effects of cell-free supernatants [CFS] from Lactobacillus acidophilus [L.acidophilus] and L. rhamnosus GG [LGG] in phorbol myristate acetate [PMA]-differentiated THP-1 cells
Materials and Methods: in this experimental study, PMA-differentiated THP-1 cells were treated with CFS from L. acidophilus, LGG and uninoculated bacterial growth media [as a control]. The expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 mRNAs were determined using real-time quantitative reverse transcription polymerase chain reaction [RTPCR]. The levels of cellular surface expression of CD147 were assessed by flow cytometry, and the gelatinolytic activity of MMP-2 and MMP-9 were determined by zymography
Results: our results showed that CFS from both L. acidophilus and LGG significantly inhibited the gene expression of MMP-9 [P=0.0011 and P=0.0005, respectively], increased the expression of TIMP-1 [P<0.0001], decreased the cell surface expression of CD147 [P=0.0307 and P=0.0054, respectively], and inhibited the gelatinolytic activity of MMP-9 [P=0.0003 and P<0.0001, respectively] in PMA-differentiated THP-1 cells. Although, MMP-2 expression and activity and TIMP-2 expression remained unchanged
Conclusion: our results indicate that CFS from L. acidophilus and LGG possess anti-inflammatory properties and can modulate the inflammatory response