RESUMEN
The aim of this study was to evaluate the tumor necrosis factor alpha [TNF-alpha] as one of cytokines produced in chronic toxoplasmosis in seropositive cases with anti-toxoplasma specific antibodies who have repeated abortions and in cases with normal delivery outcomes, compared to sero negative cases. TNF-alpha was found to be higher in seropositive cases with repeated abortion than in scronegative cases with repeated abortions and seronegative cases with normal delivery outcome
Asunto(s)
Humanos , Femenino , Toxoplasmosis , Factores de Necrosis Tumoral/sangre , Pruebas Serológicas , Inmunoglobulina G , Inmunoglobulina M , ToxoplasmaRESUMEN
ELISA and IFA tests were used to detect IgG levels in sera of experimentally infected mice with Trichinella spiralis during a period of 12 weeks post infection. A crude saline extract of muscle larvae was used for application of ELISA technique, while the intact T. spiralis muscle larvae were used as an antigen for IFAT. In both tests, T spiralis IgG antibodies could be detected one week post infection in sera of all infected mice. ELISA test showed a sensitivity of 100% during the whole period of the experiment, while its specificity was 93.3%. Meanwhile, IFA test revealed a sensitivity of 100% and a specificity of 85%