effect of Barberry Juice supplement on Prostaglandin E2 level caused by intense aerobic activity in active young girls

Background and Aim: Intense exercise can cause damages caused by oxidative stress and inflammation and, as a result, reduce exercise performance. The present study aimed at determining the effect of barberry juice supplement on the inflammation caused by intense aerobic activity in active young girls

Materials and Methods: In a quasi-experimental study, 20 young girls who had the inclusion criteria were randomly divided into two equal groups of case [receiving Barberry juice supplement] and control [receiving placebos]. The case and the control groups received 250 ml natural Barberry juice and 250ml placebos for two weeks, respectively. After the period, both of the groups took an intense aerobic exercise programme

Immediately before and after receiving Barberry juice and placebos; and also immediately after the exercise program me bordering exhaustion prostaglandin E2 [PGE2] serum level was assessed

Results: Repeated measurements revealed that PGE2 serum levels at rest state and after intense exercise activity reduced in the Barberry juice group .Besides, there were significant differences between groups the Barberry juice and placebo groups, [P

Conclusion: Generally speaking, the supplement of Barberry juice can be useful in preventing decreased performance caused by the inflammatory effects of PGE2 due to intense physical exercise

Haplotype analysis of BRCA1 intragenic markers in Iranian patients with familial breast and ovarian cancer

Background: Breast cancer is the most common malignancy in women. Breast Cancer Type 1 Susceptibility gene [BRCA1] is a tumor suppressor gene, involved in DNA damage repair and in 81% of the breast-ovarian cancer families were due to BRCA1. In some clinically investigated genes, the intragenic marker polymorphism is important and the screening of such mutations is faster by using short tandem repeat [STR] polymorphism. Individual polymorphism of STR is a good evidence for following inheritance of repeat polymorphism

Objective: The aim of this study was to evaluate three intragenic BRCA1 marker polymorphisms in families, which have two or more patients with breast/ovarian cancer in comparison to healthy women

Materials and Methods: A total of 107 breast and/or ovarian cancer patients and 93 unrelated healthy women with no clinical phenotype of any malignancy or familial cancer history constitute the study groups. Haplotyping analysis, at 3 intragenic BRCA1 microsatellite markers [D17S855, D17S1322 and D17S1323], were performed for all subject and control groups using labeled primers

Results: After fragment analysis, significance differences were observed as follows: two alleles of D17S855; allele 146 [p=0.02] and 150 [p=0.006], and two alleles of D17S1322, allele 121 [p=0.015] and 142 [p=0.043]. These differences were compared with control group. There was significance difference in 8 di/tri allelic haplotypes in present experimental subjects. Some haplotypes were observed to have approximately twice the relation risk for breast cancer

Conclusion: According to recent results, assessment of presence or absence of mentioned alleles in BRCA1 microsatellite can be used for prognosis in individuals, suspected of having or not having the breast cancer

[ effect of an endurance training period with cellular anti-aging purpose on telomerase enzyme activity in cardiac tissue and peripheral blood lymphocytes in male rats]

Introduction: The purpose of this study is to evaluate effect of moderate endurance training activity on cellular antiaging process by modulation of telomeraze enzyme content in cardiac tissue and penipheral blood eylymphocytes in male rats

Methods: Samples were rats provided by Pastear Institate of Iran. They were randomly assigned into two groups leacth composed of 8 rats

Results: 2 weeks ware allocated for adaptation to environment and changes of biological rhythm [1[st] week], and familiarity with treadmill [2[nd] week]. major protocal began after that, which includes a session of moderate daily exercise for fine days per week. Exercise continued for 16 weeks whilk the control group were sedentary. During the 1[st] eighth weeks speed of ranning on treadmill increased from 15m/min to 25m/min and reine from 15 minutes to 25 minutes progressirely, but in tensity was unchanged during the 2[nd] eighth weeks period. One day after and to the protocol, Samples were collected in fasting situation. the results showed that moderate endurance training Downloaded from jmciri.ir at 16:40 +0430 on Tuesday July 10[th] 2018 have a statistically significant effect on increasing telomerase enzyme content in cardiac tisswe [P=0/004], and lymphoeytes [p=0/004]

Conclusion: In Summany, resalts sugyested that moderate, regulaer physical activity [60-65% vozmax] have an induction effect in telomerase content and length stabilily, which in turn lead to cellular viability improvement and genomic stabilily that ploy an anti-aging role

Analysis of cytotoxic T-lymphocyte-associated antigen-4 and MMP-9 genes’ methylation and their expression profiles with risk of non-alcoholic fatty liver disease.

OBJECTIVE: To investigate the effect of promoter methylation of cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) gene and matrix metalloproteinases (MMPs) on the risk of non-alcoholic fatty liver disease (NAFLD). MATERIALS AND METHODS: CTLA-4 and MMP-9 promoter methylation were investigated using a methylation-specific polymerase chain reaction (MS-PCR) in blood samples taken from 80 NAFLD individuals and 95 healthy controls. The expression levels of CTLA-4 and MMP-9 were also assessed in 10 blood and 9 liver tissues mRNA samples from NAFLD patients. These cases were compared to the blood (n = 10) samples of healthy controls with real-time quantitative reverse transcriptase PCR. RESULTS: No significant relationship was found for methylation of CTLA-4 and MMP-9 between cases and controls. The relative expression of CTLA-4 and MMP-9 mRNA in NAFLD was not significantly different compared to healthy control samples. CONCLUSION: For the first time, our outcomes indicate that the methylation status of CTLA-4 and MMP-9 genes has no significant function on the process of NAFLD.