RESUMEN
The essential oil, isolated by hydrodistillation from fresh flowers of Spartium junceum L. collected from medicinal plant garden in Shiraz faculty of pharmacy, near Shiraz, Fars, Iran, was investigated by Gas chromatography-Mass spectrometry [GC/MS] method. The GC/MS analysis of the oil revealed the presence of 30 constituents, of which Linalool [26.18%], Tetradecanoic acid [22.83%], Camphor [13.50%], and Dodecanoic acid [13.09%] were the major, constituting altogether almost 75.60% of total composition. This is the first report of linalool as a major compound in S. junceum oil composition. For studying of 18S rRNA gene, genomic DNA content was extracted and PCR procedure was done. Sequence similarity searches were done using NCBI database and CLC sequence viewer software. The result of PCR blasted with other sequenced genes in NCBI showed 98 % similarity to the 18S small subunit rRNA of Pisum sativum [Fabaceae] and Phaseoleae environmental samples of Elev and Amb clones. The phylogenetic relationships among 71 previously reported sequences of ribosomal encoding genes from plants and this novel sequence was investigated too
RESUMEN
The contemporary trends and concepts in pharmacy are widely affected by the emergence of Nano-, Bio- or Info- technologies [NBI] as an attempt to develop different principles of medicine. This commentary is trying to make a think tank room for 50 years ahead of today's pharmacy, where the ambience of pharmacy will be affected by such technologies together with cognition [NBIC] to achieve intelligent, low adverse reaction and holistic action medicals
RESUMEN
Human Interferon beta [INF-beta] is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-beta-1b either in shake flasks or bench top bioreactor. The designed vector was constructed based upon pET-25b[+] with T7 promoter. Recombinant human beta interferon [rhINF-beta] was codon optimized and overexpressed as a soluble, N-terminal pelB fusion protein and secreted into the periplasmic space of Escherichia coli BL21 [DE3]. The sugar, Isopropyl-beta-D-thiogalactopyranoside [IPTG] was used as a chemical inducer for rhINF-beta production in the shake flasks and bench top bioreactor. Timing of beta interferon expression was controlled by using the T7 promoter. The rhINF-beta protein was extracted from periplasmic space by osmotic shock treatment and the expression of the beta interferon encoding gene in random selected transformants, was confirmed by western and dot blot methods. The maximum of product formation achieved at the OD[600nm] = 3.42 was found to be 35% of the total protein content of the strain which translates to 0.32 g L[-1]. The constructed vector could efficiently overexpress the rhINF-beta into the periplasmic space of E. coli. The obtained yield of the produced rhINF-beta was more than previous reports. The system is easily adapted to include other vectors, tags or fusions and therefore has the potential to be broadly applicable to express other recombinant proteins