RESUMEN
Background: With increasing use of antibiotics, the number and type of microbial resistance to antibiotics have been raised and at result the need for synthesis of new antimicrobials or acquire new sources of antimicrobial is indisputable. In this study the antimicrobial effects of the flowering aerial parts of Glaucium vitellinum and Gaillonia aucheri were investigated
Materials and Methods: The antimicrobial effect of mentioned extracts against Escherichia coli PTCC 1399, Pseudomonas aeruginosa PTCC 1430, Salmonella typhimurium PTCC 1639, Staphylococcus aureus PTCC 1431 and Candida albicans PTCC 5027 were evaluated by disk diffusion and the microdilution method based on CLSI protocol 2012
Results: No any zone of inhibition were detected by disk diffusion method against tested microbes for Glaucium vitellinum. After deletion of agar interference, the minimal inhibitory concentration [MIC] was determined by broth microdilution method for two plants. None of tested extracts were effective against Gram negative tested bacteria except alkaloid fraction of G. vitellinum which was effective against S. typhi. In contrast, the extracts and fractions of both plans were effective against tested gram positive bacteria especially S. aureus
Conclusion: The best result of MIC was detected for alkaloid fraction of G. vitellinum [0.09 mg/ml] Vs. G. aucheri [125 mg/ml] against S.aureus
RESUMEN
Metallo beta-lactamases [MBLs] producing Pseudomonas aeruginosa [P. aeruginosa] isolates are becoming an escalating global threat. Among the antibiotics used to treat infections associated with P. aeruginosa, resistance to carbapenem is a serious therapeutic challenge. The aim of the present study was to detect MBL-producing P. aeruginosa and to evaluate the extracts of Urtica. dioica, Carum. copticum, and Zataria multiflora on these clinical pathogens
The study was performed on hospitalized burn patients during 2014
Antibiotic susceptibility testing was assessed by broth micro dilution and disc diffusion methods. The MBLs were detected using combination disk diffusion test [CDDT] phenotypically. Then, PCR and sequencing methods were carried out to detect the MBL encoding genes. Among 83 imipenem resistant P. aeruginosa strains, 48 [57.9%] isolates were MBL-producing P. aeruginosa. PCR and sequencing methods confirmed that these strains were blaIMP-1 positive genes, whereas none were positive for blaVIM genes. Hospitalized burn patients with MBL-producing P.aeruginosa infection had 4/48 [8.3%] mortality rate. It was demonstrated that C. copticum, U. dioica, and Z. multiflora extracts had significant antibacterial effects on regular and IMP-producing P. aeruginosa strains
The prevalence of MBL-producing P. aeruginosa isolates in burn patients is generally very high. All MBL-producing strains encode the blaIMP-1 gene. Therefore, detection of MBL-producing strains has major importance in identifying drug resistance patterns in P. aeruginosa and in controlling of infections. In the current study, the extracts from C. copticum, U. dioica, and Z. multiflora had high antibacterial effects against beta-lactamase producing P. aeruginosa isolates?
RESUMEN
In this study, antimicrobial effect of total extract of Tribulus terrestris L. and its fraction containing Benzoxazine derivative [Terresoxazine] was studied for the first time in Iran. Total aqueous extract of plant was prepared and in order to separate the components, liquid/liquid extraction method with Petroleum ether was used. Liquid chromatography-mass spectrometry [LC/MS] system proved the existence of Benzoxazine derivative in the water and the third fractions. Antimicrobial effects of all extracts were examined against 10 Gram positive and negative and Candida spp. by cup plate and disk diffusion methods. Also, the minimum inhibitory concentration [MIC] was determined by micro dilution method. The total extract showed antibacterial effect on Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis. The fraction of Benzoxazine had no effect against tested microbes. MIC and minimum bactericidal concentration [MBC] determination showed that B. subtilis had the low sensitivity to the total extract. Beside, total extract in water with 1000 mg/ml concentration and total extract in Dimethyl sulfoxide [DMSO] 10% with 750 mg/ml density can be substituted to Penicillin 200 mg/ml to combat the P. aeruginosa infections. Because of antibacterial effects of Tribulus terrestris L. against both Gram negative and positive bacteria, and no antibacterial effect of the fraction containing Benzoxazine derivative, it can be concluded that antibacterial effects of the total extract is due to other active ingredients or it is because of the cumulating of different components in total extract. Therefore separation of other components of total extract and determination of their antibacterial effects can be future subjects for researches about this plant