Influence of capparis spinosa and acacia arabica on certain biochemical haemolymph parameters of Biomphalaria alexandrina

This work investigated the molluscicidal potency of dried Capparis spinosa and Acacia arabica leaves on some selected biochemical parameters of Biomphalaria alexandrina, in order to render them, physiologically, unsuitable for S. mansoni infection or at least disturb the lifecycle of the parasite within its respective snail host. The effect of the two plants on lactate dehydrogenase [LDH], 5'-nucleotidase, acid phosphatase [AP], aspartate and alanine aminotransferases [AST and ALT], alkaline phosphatase [ALP] and glucose content were studied. This work was extended to evaluate the effect of these two plants on protein profile as well as total protein [TP] content of snail's in hemolymph after 24 hours and 1 week of snails plants feeding. The study revealed that both plants induced a marked alteration in all measured parameters, where LC50 of C. spinosa after feeding at one week showed the most potent effect

Effect of allium cepa and allium sativum feeding on glucose, glycogen, protein bands profile and phenol oxidase activity in Biomphalaria alexandrina

The effect of two plants, Allium cepa and Allium sativum, on glucose and glycogen, which are the energy fuel of the snails, as well as on phenol oxidase [PO] activity after one, two, three and seven days was studied. Different protein bands were separated from the control and treated snails hemolymph after 24 hours and 1 week using SDS- electrophoresis. The study revealed that glucose and glycogen were significantly decreased after feeding on onion and garlic. Also, phenol oxidase [PO] activity was highly significantly decreased after two and seven days of feeding on garlic, while feeding on onion decreased the activity of the enzyme at all periods. Data obtained from SDS-electrophoresis showed variations in the different protein bands, which reflected the enzymatic change in the snails' tissue. So, the snails' fecundity may be reduced and in turn disturb the life cycle of schistosome parasite