Estudo do inseticida carbofurano em solo e sedimento de área de produção de arroz irrigado e controle do gorgulho aquático Oryzophagus oryzae, Taubaté, São Paulo, Brasil / Study of the insecticide carbofuran in the soil and sediment of paddy rice fields with control of the weevil Oryzophagus oryzae, in Taubaté, state of São Paulo, Brazil

Resíduos do inseticida e nematicida carbofurano e de seu principal metabólito 3-hidroxicarbofurano foram averiguados em tabuleiros de arroz irrigado e em áreas adjacentes para controle do gorgulho aquático da espécie Oryzophagus oryzae Lima, 1936. As amostras foram coletadas no período de 6/9/1999 a 4/5/2000 e analisadas por cromatografia a líquido de alta eficiência (HPLC) utilizando-se detector de fluorescência. Não foram detectados resíduos de carbofurano e de seu metabólito 3-hidroxicarbofurano dentro dos limites de determinação do método.

The presence of residues of the insecticide and nematicide carbofuran, used for weevil (Oryzophagus oryzae Lima 1936) control, and its principal metabolite 3-hydroxycarbofuran were evaluated in soil and sediment plots from paddy rice fields and adjacent areas. The samples were analyzed using a high-performance liquid chromatography and fluorescence detector. Carbofuran and 3-hydroxycarbofuran residues were not detected in any sample in amounts above the method’s detection threshold.

Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil

The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies.

Effects of terpineol on the compound action potential of the rat sciatic nerve

Terpineol, a volatile terpenoid alcohol of low toxicity, is widely used in the perfumery industry. It is an important chemical constituent of the essential oil of many plants with widespread applications in folk medicine and in aromatherapy. The effects of terpineol on the compound action potential (CAP) of rat sciatic nerve were studied. Terpineol induced a dose-dependent blockade of the CAP. At 100 æM, terpineol had no demonstrable effect. At 300 æM terpineol, peak-to-peak amplitude and conduction velocity of CAP were significantly reduced at the end of 180-min exposure of the nerve to the drug, from 3.28 + or - 0.22 mV and 33.5 + or - 7.05 m/s, respectively, to 1.91 + or - 0.51 mV and 26.2 + or - 4.55 m/s. At 600 æM, terpineol significantly reduced peak-to-peak amplitude and conduction velocity from 2.97 + or - 0.55 mV and 32.8 + or - 3.91 m/s to 0.24 + or - 0.23 mV and 2.72 + or - 2.72 m/s, respectively (N = 5). All these effects developed slowly and were reversible upon 180-min washout

Avaliacao da funcao renal em pacientes com retencao nitrogenada submetidos a urografia com bolus. / Evaluation of renal function in patients with nitrogenous retention subjected to urography with bolus

Os autores realizaram 16 urografias em pacientes portadores de insufiencia renal aguda e cronica, com altas doses de contraste iodado, obtendo imagens radiograficas diagnosticas, sem no entanto, evidenciarem alteracoes na funcao renal, baseado na dosasgem sanguinea de creatinina pre e pos exame radiologico. Tambem nao foram notados efeitos colaterais do tipo hipersensibilidade