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Mem. Inst. Oswaldo Cruz ; 107(1): 24-30, Feb. 2012. ilus, graf, tab
Artículo en Inglés | LILACS | ID: lil-612802

RESUMEN

The aim of the present study is to investigate genetic polymorphisms in Taenia solium metacestodes from different Brazilian geographical areas and to relate them to antibody recognition in serum samples of neurocysticercosis (NC) patients. Metacestodes were obtained from the Distrito Federal (DF), Bahia, Minas Gerais (MG) and São Paulo (SP) regions of Brazil. Samples of human sera from 49 individuals with NC, 68 individuals with other helminthiasis and 40 healthy volunteers were analysed (157 individuals in total). Antigens were prepared and used in enzyme-linked immunosorbent assay and western blotting assays to detect specific immunoglobulin G antibodies. Genetic distances between metacestode populations were analysed using random amplified polymorphic DNA (RAPD) analysis. Our results show that there was a higher frequency of reactivity in the DF region in the sera from NC patients (p < 0.05), while discrimination between active and inactive NC was seen only in extracts from the MG and SP regions (p < 0.05). Using RAPD, the sample from the DF region presented a greater increase compared to the other regions. A relationship between genetic polymorphisms among T. solium metacestodes from different areas in Brazil and the differences in antibody detection in patients with NC were established.


Asunto(s)
Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Animales , Niño , Preescolar , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/inmunología , ADN de Helmintos/genética , Inmunoglobulina G/sangre , Polimorfismo Genético/genética , Taenia solium/genética , Western Blotting , Brasil , Estudios de Casos y Controles , Ensayo de Inmunoadsorción Enzimática , Geografía , Técnica del ADN Polimorfo Amplificado Aleatorio , Taenia solium/inmunología , Taenia solium/aislamiento & purificación
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