Rubiadin exerts an acute and chronic anti-inflammatory effect in rodents

Abstract: Rubiadin is identified as a bioactive anthraquinone that exists in some quinone rich plants. The current research was carried out to evaluate the potential anti-inflammatory impact of Rubiadin in acute and chronic inflammation test models in rodents. The anti-inflammatory activity of Rubiadin was examined in cotton pellet-induced granuloma and carrageenan-induced edema as chronic and acute inflammation models in rats. TNF- level and histopathological changes were assessed using sampled foot tissue of rat in the acute model. Also, the IL-1 level was assessed in the chronic model. One-way ANOVA (post hoc Tukeys) analysis was used for comparing the groups. Rubiadin (0.5 mg/kg, i.p.) induced a significant reduction in TNF level and the paw edema compared to the control group in carrageenan test. Also, it was observed that the anti-inflammatory activity of Rubiadin (0.5 mg/kg, i.p.) is comparable to mefenamic acid (30 mg/kg, i.p.) as the standard drug. Rubiadin was effective in granuloma induced by cotton pellet concerning the granuloma and transudate formation amount. Rubiadins anti-inflammatory effects were associated with a significant IL-1 decrease in this model. The results suggest that Rubiadin as a natural compound can possess significant peripheral anti-inflammatory impacts.

Resumo A rubiadina é identificada como uma antraquinona bioativa que existe em algumas plantas ricas em quinonas. A presente pesquisa foi realizada para avaliar o potencial impacto anti-inflamatório da rubiadina em modelos de teste de inflamação aguda e crônica em roedores. A atividade anti-inflamatória da rubiadina foi examinada em granuloma induzido por pellet de algodão e edema induzido por carragenina como modelos de inflamação crônica e aguda em ratos. O nível de TNF- e as alterações histopatológicas foram avaliados usando amostra de tecido do pé de rato no modelo agudo. Além disso, o nível de IL-1 foi avaliado no modelo crônico. A análise ANOVA de uma via (post hoc de Tukey) foi usada para comparar os grupos. A rubiadina (0,5 mg / kg, i.p.) induziu uma redução significativa no nível de TNF e no edema da pata em comparação com o grupo de controle no teste de carragenina. Além disso, foi observado que a atividade anti-inflamatória da rubiadina (0,5 mg / kg, i.p.) é comparável ao ácido mefenâmico (30 mg/kg, i.p.) como o fármaco padrão. A rubiadina foi eficaz no granuloma induzido por pellet de algodão no que diz respeito à quantidade de granuloma e formação de transudato. Os efeitos anti-inflamatórios da rubiadina foram associados a uma redução significativa de IL-1 nesse modelo. Os resultados sugerem que a rubiadina como um composto natural pode ter impactos anti-inflamatórios periféricos significativos.

Insulin replacement prevents the acquisition but not the expression of morphine-induced conditioned place preference in streptozotocin-induced diabetic rats

Abstract Insulin receptors have distributed in all brain regions, including the nucleus Accumbens (NAc), and where is implicated in the reward properties of drugs. It is well known that insulin signaling can regulate dopamine release. Therefore, in the present study, we tried to examine the effect of insulin replacement on the acquisition and expression of morphine-induced conditioned place preference (CPP) in diabetic rats. Forty-eight male Wistar rats were divided into two non-diabetic (Naïve) and diabetic groups rendered by a single injection of streptozotocin (STZ). These groups separately received insulin (10U/kg) or saline (1 ml/kg) one hour prior to morphine administration (5mg/kg;s.c.) during conditioning days (acquisition phase) or post-conditioning day (expression phase) in the CPP paradigm. In this paradigm, conditioning score (CS) and locomotion activity were recorded by Ethovision. The STZ-induced diabetic rats displayed higher CS compared to naïve rats (P<0.05). This effect was abolished in all diabetic rats that received insulin during conditioning days but not the expression phase. This study has provided evidence that insulin plays a modulatory role in morphine-induced CPP, and insulin replacement during the acquisition phase could reduce the rewarding properties of morphine in diabetes conditions through a possible modulating effect on dopamine release in the NAc region

Evaluating the related factors to healthimprovement style and the value of health among middle aged women referring to comprehensive health centers of Isfahan in 2016 / Evaluar los factores relacionados con el estilo de mejora de la salud y el valor de la salud entre las mujeres de mediana edad que se refieren a los centros de salud integrales de Isfahan en 2016

Introducción. El presente estudio se realizó para evaluar los factores relacionados con el estilo de vida de mejora de la salud y el valor de la salud entre las mujeres de mediana edad. Material y Métodos: El presente estudio fue una investigación descriptiva-correlacional que se realizó con la participación de 278 mujeres de mediana edad que remitieron a los centros de salud de Isfahan y fueron seleccionadas al azar en 2016. Los datos se recopilaron mediante el cuestionario de características demográficas, Walker's Health Promoting Lifestyle Profile II (HPLPII) y un cuestionario realizado por un investigador para el valor de la salud que fueron completados por los participantes. Los datos se analizaron utilizando el software SPSS con prueba de coeficiente de correlación de Pearson, análisis de regresión lineal, prueba de Mann-Whitney y prueba de Wilcoxon. Resultados: De acuerdo con los resultados del presente estudio, hubo una relación directa entre la puntuación del valor de la salud y la puntuación total del estilo de vida y las puntuaciones de todos sus dominios (p <0,05). Hubo una relación significativa entre el empleo de mujeres de mediana edad y los dominios de las relaciones personales y el estilo de vida de crecimiento espiritual y también entre las enfermedades de fondo y el estrés físico y el manejo del estrés del estilo de vida para mejorar la salud (p <0,05). También se observó una relación significativa entre la edad de las mujeres de mediana edad y el valor de la salud y el dominio de la responsabilidad del estilo de vida de mejora de la salud (p <0,05). Conclusiones: Para mejorar el nivel de mejora de la salud y el valor de los estilos de vida de salud entre este grupo de mujeres, es necesario prestar atención a cuestiones tales como el apoyo, la facilitación y los factores personales y sociales en los programas de atención médica para estas mujeres

Introducction. The present study was conducted to evaluate the related factors to health-improvement lifestyle and value of health among middle aged women. Material and Methods: The present study was a descriptivecorrelational research that was conducted with participation of 278 middle aged women who referred to the health centers of Isfahan and were selected randomly in 2016. Data were collected using demographic characteristics questionnaire, Walker's Health Promoting Lifestyle Profile II (HPLPII) and a researcher-made questionnaire for the value of health that were completed by the participants. Data were analyzed using SPSS software with Pearson correlation coefficient test, linear regression analysis, Mann-Whitney test and Wilcoxon test. Results: According to the results of the present study there was a direct relation between the score of value of health and the total score of lifestyle and the scores all of its domains (p < 0.05). There was a significant relation between the employment of middle aged women and the domains of personal relationships and spiritual growth lifestyle and also between background diseases and physical stress and stress management of the healthimprovement lifestyle (p < 0.05). Also a significant relation was observed between the age of the middle aged women and the value of health and the domain of responsibility of the heath-improvement lifestyle (p < 0.05). Conclusions: To enhance the level of the health-improvement and value of health lifestyles among this group of women it is necessary to pay attention to matters such as support, facilitation and personal-social factors in the healthcare programs for these women

In vivo analgesic and anti-inflammatory effects of the essential oil from Tanacetum balsamita L

Tanacetum balsamita locally called Shahesparam is an aromatic plant that grows widely in Azerbaijan Province, Iran. Due to the widespread use of T. balsamita as a pain killer and relief of inflammatory based disorders in Iranian folk medicine and considering the high content of essential oil in T. balsamita aerial parts, we were prompted to investigate the anti-nociceptive and anti-inflammatory properties of T. balsamita essential oil (TBEO) for the first time. The carrageenan-induced Paw Edema was used for inflammation evaluation in rat, and hot-plate method was used for pain assessment in mice. Different doses of TBEO were administered. Morphine and Mefenamic acid were used as the standard drugs in anti-nociceptive and anti-inflammatory evaluation tests, respectively. TBEO (100 mg/kg) showed significantly anti-nociceptive activity in hot-plate test. The anti-inflammatory activity of TBEO was found to be more than mefenamic acid. The studied oil was analyzed by GC and GC-MS. The major component of the oil was characterized as carvone (39.8%) which might be responsible for the observed activities. The results suggested that TBEO possessed biologically active constituents that had significant analgesic and anti-inflammatory effects which support the ethno-medicinal claims of the plant application in the management of pain and inflammation.