Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Añadir filtros








Intervalo de año
1.
Biol. Res ; 46(3): 289-294, 2013. ilus, graf, tab
Artículo en Inglés | LILACS | ID: lil-692196

RESUMEN

Phototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere. These microvilli encompass the phototransduction proteins, most of which are transmembrane and exclusively rhabdomeric. Rhabdomere membrane lipids play a crucial role in the activation of the transient receptor potential ionic channels (TRP and TRPL) responsible for initiating the photoresponse. Despite its importance, rhabdomere lipid composition has not been established. We developed a novel preparation enriched in rhabdomere membranes to perform a thorough characterization of the lipidomics of Drosophila rhabdomeres. Isolated eyes (500) were homogenized and subjected to a differential centrifugation protocol that generates a fraction enriched in rhabdomere membrane. Lipids extracted from this preparation were identified and quantified by gas chromatography coupled to mass spectrometry. We found an abundance of low sterol esters (C16:0, C18:0), highly abundant and diverse triglycerides, free fatty acids, a moderate variety of mono and diacyglycerols (C:16:0, 18:0, C18:1) and abundant phospholipids (principally C18:2). This preparation opens a new avenue for investigating essential aspects of phototransduction.


Asunto(s)
Animales , Proteínas de Drosophila/química , Drosophila melanogaster/química , Ácidos Grasos/análisis , Microvellosidades/química , Células Fotorreceptoras de Invertebrados/química , Canales de Potencial de Receptor Transitorio/química , Proteínas de Drosophila/análisis , Fototransducción/fisiología , Transporte de Proteínas/fisiología , Canales de Potencial de Receptor Transitorio/análisis
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA