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Zhongguo Zhong Yao Za Zhi ; (24): 1265-1268, 2005.
Artículo en Chino | WPRIM | ID: wpr-239705

RESUMEN

<p><b>OBJECTIVE</b>To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.</p><p><b>METHOD</b>The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.</p><p><b>RESULT</b>The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.</p><p><b>CONCLUSION</b>The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.</p>


Asunto(s)
Acetofenonas , Cromatografía Líquida de Alta Presión , Métodos , Ácido Gálico , Paeonia , Química , Corteza de la Planta , Química , Plantas Medicinales , Química , Control de Calidad
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