effects of Adiantum capillus-veneris on wound healing: an experimental in vitro evaluation

A lot of remedies, mostly plant based, were mentioned in the Persian old pharmacopoeias for promoting of burn and wound healing and tissue repairing. The efficacy of most of these old remedies is unexplored till now. Adiantum capillus-veneris from Adiantaceae family is one of them that was used to treating of some kinds of chronic wounds. Methanol extract was fractionated to four different partitions that is, hexane, ethyl acetate, n-butanol, and aqueous. The potential of A. capillus-veneris fractions in wound healing or prevention of chronic wounds were evaluated through angiogenesis and fibroblast proliferation, in addition to in vitrotests for protection against damage to fibroblasts by oxygen free radicals. The aqueous part of A. capillus-veneris promoted significant angiogenesis [P < 0.05] through both capillary-like tubular formations and proliferation of endothelial cells in vitro. In addition, in the tests for protection against damage to fibroblasts by oxygen free radicals, aqueous and butanol fractions showed significant protective effects in the concentrations 50, and 500 microg/ml [P < 0.05] in comparison with a control group. In the toxicity testing, it showed weak irritation in the Hen's egg test chorioallantoic membrane [CAM] bioassay at the vascular level on the CAM of the chicken and no significant cytotoxicity in the MTT assays on normal human dermal fibroblasts. Angiogenic effects and protective effects against oxygen free radicals suggested aqueous partition of A. capillus-veneris local application for prevention of late-radiation-induced injuries after radiation therapy and healing of external wounds similar to bedsores and burns

Triterpenes and steroids from euphorbia denticulata lam. with anti-herpes symplex virus activity

In this research, dried acetoneichloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3beta,25-diol, cycloart-23E-ene-3beta,25-diol, ergosta-8,24-dien-3-ol [obtusifoliol] and beta-sitosterol which were reported for the first time from this plant. The structure of these compounds was elucidated by NMR and mass spectroscopic methods. The MTS assay was used to determine the toxicity and antiviral activity of betulin and [3/beta,23E]-cycloarta-23-ene-3,25-diol. Betulin showed anti-HSV-1 activity with EC50 value of 84.37 +/- 0.02 microg/mL, and toxicity on normal vero cells with CC50 value of 660.718 +/- 0.072 microg/mL. [3beta,23E]-Cycloarta-23-ene-3,25-diol showed antiviral effect with EC50 value of 86.63 +/- 0.03 microg/mL, and toxicity with CC50 value of 1089.2 H0.25 microg/mL. The results revealed that these two compounds have the antiviral activity far below the CC50 value with selectivity index [CC50/EC50] values of 7.83, and 12.57, respectively

Flavonol glycosides from euphorbia microsciadia bioss. with their immunomodulatory activities

Four known flavonoids: quercetin 3-O-beta-D-rutinoside [Q3Rut], myricetin 3-O-betaD-galactopyranoside [M3Gal], quercetin 3-O-beta-D-galactopyranoside [Q3Gal] and quercetin 3-Obeta-D-glucopyranoside [Q3Glc], for the first time were isolated from aerial parts of Euphorbia microsciadia. The chemical structure of them was elucidated on the basis of 1 and 2 D-NMR spectra and different spectroscopic techniques. The immunomodulatory activities of isolated compounds were compared using standard T-cell proliferation assay. These data showed that lymphocyte suppression activity of flavonoids [1-4] were comparatively lower than prednisolon as a standard drug. Immunosuppressive activity of flavonoids with hydroxyl groups at both 3'-and 4'-positions in their B-ring [Q3Gal] were more than those with 3'-, 4'-and 5'-hydroxyl substitution [M3Gal]. In these compounds, Q3Gal showed the most inhibitory activity, whereas M3Gal showed the least lymphocyte antiprolifeartive activity

Phytochemical analysis and metal-chelation activity of achillea tenuifolia lam

Achillea tenuifolia Lam. [Asteraceae] afforded a dichloromethane fraction from which three known compounds beta-sitosterol [compound1], 5-hydroxy, 4',6,7- trimethoxy flavone [salvigenin compound 2], and methyl-gallate [compound 3] were isolated for the first time. The structure of isolated compounds was elucidated by different spectroscopic methods. Applying the molar-ratio method, the complexation of salvigenin with Fe [III], Cu[II] and Zn[II], the most abundant type of metal ions in the body, were then evaluated. It was determined that stoichiometric ratio of salvigenin with these cations were as Fe[Salvigenin][2] [H2O][2] and Cu[Salvigenin][2][H2O][2] in methanolic solution without pH control, while zinc ions didn't form significant complexes. The results were confirmed more, by computational molecular modeling of the structure of proposed ligand-complexes by semi-imperical PM3 calculations, which determined negative heat of formation for the complexes Fe[III] and Cu[II] ions as -689.7 and -573.5, respectively and proposed chelating affinity of salvigenin in the following order: Fe[III] > Cu[II] >> Zn[II]