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1.
Indian J Exp Biol ; 2014 Jan; 52(1): 80-88
Artículo en Inglés | IMSEAR | ID: sea-150336

RESUMEN

Brinjal (Solanum melongena L.) var. Mattu Gulla (MG) and var. Perampalli Gulla (PG) are unique varieties with distinct flavour cultivated in Udupi, Karnataka State, and are exposed to several biotic and abiotic stresses. An efficient and reproducible in vitro regeneration method is required to expedite the manipulation of these brinjal varieties to cope up with stress by tissue culture and gene transfer methods. The present study, reports a rapid and efficient in vitro regeneration protocol for these two varieties. The in vitro growth response was studied on Murashige and Skoog (MS) medium supplemented with 2, 4-D, BAP and IAA, and the plantlets were regenerated efficiently from callus cultures of leaf, cotyledon and hypocotyl explants. Among the three explants, the hypocotyl explants were found to have better callus induction and multiple shoot regeneration. High frequency of shoot initiation was achieved from hypocotyl derived calluses in MS media with 2.0 mg/L BAP and 0.5 mg/L IAA in MG and PG. Efficient and rapid shoot proliferation, and elongation were noted in MS medium with 1.0 mg/L BAP and 0.3 mg/L GA3. The in vitro regenerated shoots produced healthy roots when they were cultured on MS medium supplemented with 0.5 mg/L IBA. A significant difference was observed in percentage of callus induction, number of shoots per callus, shoot elongation and number of hardened plantlets of MG and PG. MG showed maximum response in all stages of culture than PG. Hardening of plantlets in tissue culture was achieved in three weeks. The hardened plantlets were grown in pots for further acclimatization in green house and finally transplanted to experimental garden where they developed into flowering plants and produced mature fruits with viable seeds.


Asunto(s)
Técnicas de Cultivo de Célula , Cotiledón/citología , Cotiledón/crecimiento & desarrollo , Medios de Cultivo , India , Reguladores del Crecimiento de las Plantas/farmacología , Hojas de la Planta/citología , Hojas de la Planta/crecimiento & desarrollo , Raíces de Plantas/citología , Raíces de Plantas/crecimiento & desarrollo , Brotes de la Planta/citología , Brotes de la Planta/crecimiento & desarrollo , Regeneración/fisiología , Semillas/citología , Semillas/crecimiento & desarrollo , Solanum melongena/crecimiento & desarrollo
2.
J Environ Biol ; 2001 Oct; 22(4): 237-42
Artículo en Inglés | IMSEAR | ID: sea-113517

RESUMEN

The effect of sago and sugar factory effluents was studied on Gossypium hirsutum L. var. MCU 5 and MCU 11. Plants were irrigated with 0, 25, 50, 75 and 100% of effluents of both factories. At lower concentration (25%) of sugar factory effluents had stimulatory effect on all biochemical contents observed. Moreover, all concentration of sago factory effluents were found to have inhibitory effect on all biochemical contents except proline content which increased with increasing concentration of both the effluents. Plants growing on adjacent to sago and sugar factories or they irrigated with such type of polluted water, may accumulate the heavy metals found in both the effluents, at higher levels in plant products and if consumed may have similar effect on living organisms.


Asunto(s)
Exposición a Riesgos Ambientales , Gossypium/fisiología , Residuos Industriales/efectos adversos , Industrias , Metales Pesados/efectos adversos , Eliminación de Residuos Líquidos , Contaminantes Químicos del Agua/efectos adversos
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