RESUMEN
Helicobacter pylori [H. pylori] is a gram-negative, microaerophilic bacterium that cause the stomach infection in more than 50% of human population worldwide. The aim of this study was to examine the possibility of anti-H. pylori immunoglobulins Y [IgYs] production in quails and evaluate the effect of the different methods of immunization on titers of IgY in egg yolks. Whole cell bacterial antigen was used for immunization of quails. Forty Japanese quails [Coturnix japonica] were divided into four groups. The first group intramuscularly immunized with one dose of antigen [3 × 108 inactivated bacteria] whereas the second group injected with half dose. Third group administered orally. Yolk IgY was isolated using precipitation method of water dilution combined with chloroform. Dot-blot and ELISA [enzyme-linked immunosorbent assay] were used for determining the specificity and quantifying the titer of IgY in egg yolks. Results showed that quails as well as chickens are able to produce anti-H. pylori IgY. Quails antibodies have high titer and specificity that can be used in therapeutic and research purposes. This study indicated that higher amounts of antigen can not develop higher titer of IgY and injection is not necessary for efficient immunization of the quail against H. pylori
RESUMEN
Introduction: Hypnosis is one of the psychological approaches that used in multiple sclerosis patients. The effect of hypnosis depends on individual hypnotic susceptibility. Hypnotic susceptibility is degree of response to the hypnotic suggestions and is a stable personal characteristic. The aim of present study was to determine correlation between personal characteristic and mental health with the degree of hypnotic susceptibility
Methods: This correlation study was carried out on 60 women with multiple sclerosis referring to the MS Society of Urmia. The data collection tools that used here include demographic questionnaire, General health questionnaire [GHQ] and Stanford hypnotic susceptibility scale [SHSS]
Results: In the investigation of the correlation between the variables and the hypnotic susceptibility of the samples, the Pearson correlation test result showed a statistical correlation between age and the degree of hypnotic susceptibility[r=-0.29, p value=0.023]. In investigation of the correlation between the level of education and hypnotic susceptibility, the independent t-test results showed no statistically significant correlation between the two variables [p value>0.05]. The results of ANOVA test also showed that there is no significant correlation between employment status of the samples and their hypnotic susceptibility [p value = 0.420]. In analysis of relation between mental health and the degree of hypnotic susceptibility, Pearson correlation test results showed weak correlation between two variables [r=0.089, p value=0.499]
Conclusion: In general it can be concluded that among the personal characteristics, the only significant correlation is between age and degree of hypnotic susceptibility of samples and hypnotic susceptibility is not influenced by other personal characteristics. It can also be concluded that hypnotherapy is a effective and safe treatment which could well be used in MS patients