Degradation of shrimp-shell waste and production of chitinase by bacillus amyloliquefaciens under some cultural conditions

The study of chitinase production by Bacillus amyloliquefaciens grown on shrimp-shell waste as the sole carbon source showed that chitinase production with maximum enzyme activity was reached when 2% inoculum of 24 h age seed culture was used. The inoculated medium contained [g/I] shrimpshell waste, 80; NH[4]CI, 1.2: KH[2]PO [4]; K[2]HPO[4], 1.6; MgSO[4].7H[2]O, 0.1: ZnSO[4]. 7H[2]O, 0.1; ZnSO[4] .7H[2]O. FeCI[3]. 6H[2]O, 0.01; CaCI[2]2]H[2]O, 0.02 at pH 7 and the culture shaken at 30°C for 36 hours

Some factors influencing chitinase production by bacillus amyloliquefaciens

The effect of several analogues and breakdown products of chitin on the iodsetioa of the chitinase of B. amyloliquefaciens was examined. Glucose. N -acetyl-glucosamine and chitobiose inhibited the enzyme productivity. The effect of the addition of some natural extracts was also investigated and the maximum enzyme activity was produced with 2% sand extract. A simplified medium composed of 8% shrimp-shell waste and 80% sand extract yielded an enzyme of highest activity. Reaction conditions supporting best activity sum achieved using 5-7-5mg swollen chitin in 0.03 M phosphate buffer at pH 5.916.98; enzyme concentration of 11.35mg protein/reaction mixture and incubating the mixture at 45°C for 60 min