Comparative investigation of klebsiella clinical isolates including klebsiella planticola and klebsiella terrigena

Ninety-one Klebsiella clinical isolates were classified into Klebsiella pneumoniae [25], Klebsiella ozanae [21], Klebsiella rhinoscleromatis [1], Klebsiella oxytoca [26], Klebsiella planticola [7] and Klebsiella terrigena [11], using differentiating biochemical tests. The susceptibility of these isolates to 18 chemotherapeutic antimicrobial agents was tested. Most of the isolates were resistant to ampicillin, carbenicillin, chloramphenicol, tetracycline, streptomycin and contrimoxazole. Some of the strains were resistant to as many as 15 antimicrobial agents including third generation cephalosporins. On the contrary, all the isolates were sensitive to ofloxacin. The pattern of antibiotic resistance and beta lactamase production by K. planticola and K. terrigena isolates was determined. Heterogeneous resistance patterns were obtained. Both the rate of conjugation and the pattern of antibiotic resistance of transconjugants were evaluated

Single step high rifampin resistance in clinical isolates of methicillin-resistant staphylococcus aureus and its prevention by erythromycin combination

The emergence of resistance to the potent antistaphylococcal antibiotic rifampin [RIF] has been investigated against 6 methicillin- and ampicillin-resistant Staphylococcus aureus clinical isolates using turbidimetric and viable count techniques. Single step high level resistance was developed rapidly due to induction of mutants resistant to suprapharmacological levels [1000 times the minimum inhibitory concentration, MIC] of RIF irrespective of the concentration of RIF present. Erythromycin [ERY] at an MIC level effectively prevented RIF resistance. Thus, the RIF-ERY combination warrants further evaluation in the therapy of staphylococcal infections, especially those resistant to B-lactam antibiotics

Influence of some preservatives on the bioactivity of digestive enzymes

The effect of seven commonly used preservatives on the biological activity of chymotrypsin, diastase, pepsin, pancreatin and trypsin were studied by the radial diffusion technique. The proteolytic activities of papain, pancreatin and chymotrypsin were reduced by the tested preservatives in descending order, while those of pepsin and trypsin were unaffected. Reductions in the amylolytic activity of pancreatin and diastase varied according to the preservative tested

Microorganisms-digestive enzymes interactions

The viability of B. subtilis, E. coli, Ps. aeruginosa and C. albicans in 0.5% sterile solutions of diastase, pancreatin, papain, pepsin and trypsin during a period of 7 days was followed by the viable count technique. Marked growth of these organisms took place, though the extent of which varied according to the enzyme. At 4C, the enzymes suffered practically no alteration in their amylolytic or proteolytic activities. Storage at 25C resulted in significant reductions of amylolytic activity [0.3-89.7%] and proteolytic activity [0-77%], depending on the enzyme-organism system. B. subtilis and C. albicans exerted, in general, the highest inactivation of the enzymes. On the other h and, Ps. aeruginosa produced little effect