Isolation of stem cells from the mid gut epithelium of culex pipiens mosquitoes [diptera: culicidae]

Stem cells are undifferentiated cells that remarkable ability to self-renew and produce differentiated cells that support normal development and tissue homeostasis. The authors isolated spherical stem cells from the midgut epithelium of the late third larval instars of Culex pipiens mosquitoes. In vitro, cultivated epithelial stem cells were able to proliferate and differentiate in fresh culture for 48 hr in absence of 20- hydroxyecdysone [20-E]. The stem cells were the most common cells in the initial culture. One hundred of epithelial stem cells had been cultured. After 24 hr became 132 cells, 78% was undifferentiated stem cells and 8.3% and 13.7% was enterocytes and goblet cells respectively. This ratio slightly decreased after de 48 hr to be 74% undifferentiated stem cells, 4.5% enterocytes and 5.6% goblet cells. After 72 hr the undifferentiated stem cells were not able to proliferate .The most differentiated goblet and enterocytes survived few days then died

Distinguishing the two forms of egyptian aedes [ochlerotatus] caspius palias species [diptera: culicidae] by ultra structure micrographs of eggs

Ultrastructure of the two forms autogenous and anautogenous eggs of Aedes [Ochlerotatus] caspius of Egypt are described using Scanning Electron Microscope [SEM]. The eggs of the two forms are slightly boat shape with quite difference in width. Chorionic cells of the ventral surface are ultimately different in both forms in shape, width of reticulum, number and size of tubercles. The chorionic cells of the autogenous form's egg are elongate, narrow and almost curved with unusually wide, outer reticulum contain 2 - 13 large tubercles along with a few number in small size. However, the anautogenous form's egg, the chorionic cells of the ventral surface fairly distinct, very regular in outline with thin reticulum and usually hexagonal, each cell contain one or two large tubercles with many small scattered peripheral tubercles. Fine structure micrographic work of eggs of the Egyptian Ae. caspius provides new morphological evidence that both autogenous and unautogenous forms are certainly different and suggests that those forms are two distinct species