RESUMEN
Objective To investigate the prevention of distant multiple organ dysfunction caused by lower limb ischemia/reperfusion with taurine.Methods Wistar rats were randomly divided into 3 groups.(1)Reperfusion group:rats underwent 2-hour bilateral artery femoralis of hind limbs ischemia and followed by 5 hours reperfusion.(2)Taurine group:each rat was oraled 20% taurine 3ml every day for 7 days and underwent 2hrs ischemia followed by 5 hrs reperfusion.(3)Control group:sham animals didn't undergo ischemia.The count of leukocyte,myocardial and liver enzymes were measured.Ultrastructural pathological change of distant organs were observed.Superoxide dismutase(SOD) of erythrocyte,malondialdehyde(MDA) glutathione(GSH) and tumor necrosis factor(TNF) in tissue homogenization was assayed.Results The count of leukocyte was decreased significantly in reperfusion group,myocardial and liver enzymes were significantly increased compared with control group ultrastructural pathological change of extensive injury of distant multiple organs was occurred in perfusion group.SOD of erythrocyte and GSH in serum were decreased compared with control group,while MDA and tumor necrosis factor in tissue homogenization of liver and lung were significantly increased.The pathological changes of taurine group was obviously alleviate.Conclusion Limb ischemia/reperfusion can produce quantities of oxygen free radicals and TNF which were released by activated polymornuclear leukocytes.This made the body plunge into systemic inflammation response syndrome and induce distant multiple organ dysfunction.Taurine can prevent it obviously.