High performance liquid chromatography:a rapid isocratic method for determination of energy compounds in myocardial tissue / 中国病理生理杂志

To determine energy compounds in myocardial tissue simultaneously, we determined standard Creatine(Cr), Creatine phosphate(PCr), Hypoxanthine(Hypo), Nicotinamide adenine dinucleotide (NAD), Adenosine monophosphate (AMP), Adenosine diphosphate (ADP), Adenosine triphosphate (ATP), Cyclic-adenosine monophosphate(cAMP), and other compounds related to energy metabolism by a rapid isocratic method.The result showed that standard curves of the eight compounds were linear and passed through the origo for all examined concentrations (r=0.999).A recovery (88.1～117.6%) and replication (CV%=0.97～3.78%) for the present method was found to be accord with methology request.