new modified method for the detection of antibody producing cells during parasitic infections

A modified method for detecting antibody producing cells [APC] was described in this work The method depends on spreading the cell mixture prepared for the assay in a single layer on a gelatin cusion without using a solidifying medium. The method was proved to be highly sensitive and less costly. The method was applied to detect APC during infection with H nana. Infected animals showed fewer APC in their splenocytes when compared with a control group

new stain for parasitic protozoa and helminths ova extracted from the roots of Furwa plant Rubia Tinctorum L.

In this work, a new highly efficient stain was discovered to stain different parasitic protozoa and most of the common helminths ova. The stain was extracted from the roots of the wild plant Fuwa [Rubia], and both watery and alcoholic extracts were examined for their ability to stain different parasitic protozoa [both cysts and trophozoites] and helminths ova. The stain has proved to be highly efficient and it has been suggested that the new stain could be used as an alternative to the conventional stains such as iodine and trichrome. No obvious differences were noticed between the alcoholic and watery extracts of the new stain, therefore it was recommended to use the watery solution of the stain [in sa1ine] at a concentration of 6% [wt/vol.] for both routein and teaching purposes. The stain. can be easily obtained and it is much less costly than other conventional stains