Cell death pattern in cerebellum neurons infected with toxoplasma gondii

There is a demand for studying the role of Toxoplasma gondii in cell death seeking aiding prevention of the disease. The neuro-pathological changes in the cerebellum cortex in case of acquired toxoplasmosis had been studied. Adult Balb C mice were infected by intra peritoneal injection of T. gondii RH strain. Immuno-histochemical expression of pro apoptotic marker Bax had been applied in parallel with Hematoxylin and Eosin stain to study the layers of cerebellum cortex. The focal necrosis in the cerebellum was expressed. Necrosis was explained on the basis of hypoxic ischemia resulting from existing vasculitis followed the infection. Purkinje cell layer was markedly affected in the form of disfiguring and focal loss of cells with apoptotic and necrotic changes. Thinning of both the molecular and internal granular layers was recorded morphometricly. Morphometric study reveals non significant change in the ratio between the viable to non viable cells in all cerebellum layers among experimental and control groups though the Purkinje cell layer was mostly affected. Statistical significant changes in depth proportion of molecular layer: Internal granular [ML: IGL] layers was noted in experimental and control group [p 0.05]. Bax expression was not coexisting with the result of H and E stained cells. The hypothesis emphasizes that toxoplasmosis resist apoptosis seeking its benefit, and apoptosis followed toxoplasmosis may be due to another protein rather than Bax

Farm animals' fascioliasis in Ezbat El-Bakly [tamyia center] Al-Fayoum governorate

To continue the study on fascioliasis in Tamyia Center, some farm animals were investigated for natural infection with Fasciola species by stool examination. The results showed 40% infection in sheep, 20% in buffalos, 6.7% in donkeys and zero% in horses. The overall percentage of infection was 25.5. The sheep [total dose 1800 mg] and the donkey [total dose [4500 mg.] were successfully treated with Mirajid R. On the other hand, one buffalo was successfully treated by a total dose 7500mg, the second one did not cured, but the eggs deposited per gm markedly decreased