Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Añadir filtros








Intervalo de año
1.
Braz. j. med. biol. res ; 29(6): 763-7, jun. 1996. ilus, tab
Artículo en Inglés | LILACS | ID: lil-181410

RESUMEN

The presence of antibody isotypes (IgG, IgA and IgM) to streptolysin O was determined by dot ELISA in 222 serum samples from patients with different levels of anti-streptolysin O (SLO) antibodies as measured by the neutralizing assay (NA), from patients with diseases not related to nonsuppurative complications of Streptococcus pyogenes infection, and from clinically healthy individuals. Immunoglobulin G antibodies were found in 72 per cent of sera from patients with SLO antibodies higher than 333 Todd units (TU), and IgA antibodies were also detected in 53 per cent, but no IgM antibodies were demonstrable. High copositivity (0.94), conegativity (0.97), and positive (0.96) and negative (0.96) predictive values were observed when IgG and IgA findings were combined. The dot ELISA gave highly reproducible results. The present data suggest that the assay may be of practical value for routine detection of SLO antibodies when employed with an anti-human immunoglobulin light chain peroxidase conjugate.


Asunto(s)
Humanos , Inmunoglobulina A/análisis , Inmunoglobulina G/análisis , Inmunoglobulina M/análisis , Infecciones Estreptocócicas/diagnóstico , Streptococcus pyogenes , Estreptolisinas , Ensayo de Inmunoadsorción Enzimática , Valor Predictivo de las Pruebas , Reproducibilidad de los Resultados , Pruebas de Neutralización/métodos
2.
Braz. j. med. biol. res ; 24(5): 471-83, 1991. tab
Artículo en Inglés | LILACS | ID: lil-99479

RESUMEN

Diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) was standardized and evaluated for the diagnosis of Chagas'disease in comparison with the conventional serological tests indirect immunofluorescence (IFI), passive hemagglutination (PHA) and complement fixation (CF). A total of 236 serum samples positive and negative for the serodiagnosis of Chagas'disease were studied. The group included 50 serum samples serologically positive for leishmaniasis and 36 positive for malaria. The best diagnostic performance of DIG-ELISA was observed when serum samples were diluted to 1:8 and a diameter of zero mm (no color) was taken as the cut-off. Under these conditions, the relative indices of sensitivity, specificity and agreement were 100%. High positive correlation coeficients were obtained between DIG-ELISA and IFI (r1=0.9010), PHA (r2=0.8943) and CF (r3=0.8269). We conclude that DIG-ELISA provides an alternative technique for screening chagasic infections, as well as for seroepidemiological surveys mainly because it is simple, easy to carry out and does not require expensive equipment


Asunto(s)
Enfermedad de Chagas/diagnóstico , Ensayo de Inmunoadsorción Enzimática/métodos , Inmunodifusión/métodos , Anticuerpos Antiprotozoarios/sangre , Pruebas de Fijación del Complemento , Técnica del Anticuerpo Fluorescente , Pruebas de Hemaglutinación , Inmunoglobulina G/sangre , Leishmaniasis/diagnóstico , Malaria/diagnóstico , Análisis de Regresión , Sensibilidad y Especificidad , Pruebas Serológicas , Trypanosoma cruzi/inmunología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA