Determination of several environmental contaminants in human body / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To detect common environmental pollutants in human body.</p><p><b>METHODS</b>Urine samples were collected from 80 healthy subjects. Chromatography mass spectrometry (GC-MS), HPLC and ELISA were applied to detect several common environmental pollutants in urine samples.</p><p><b>RESULTS</b>DBP and methylbenzene were present in 75.3% and 41.2% of urine samples. The methanal and AFM1 were found in most of urine samples (approximately 91≊97%). By contrast, PCBs, CPZ, 4, 5-DCC were found in less than 5 samples, but there was no TMT detected.</p><p><b>CONCLUSION</b>Some of the environmental pollutants including carcinogens are detected in urine samples in this study.</p>

Purification of arsenic-binding proteins in hamster plasma after oral administration of arsenite / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To purify the arsenic-binding proteins (As-BP) in hamster plasma after a single oral administration of arsenite (iAs(III)).</p><p><b>METHODS</b>Arsenite was given to hamsters in a single dose. Three types of HPLC columns, size exclusion, gel filtration and anion exchange columns, combined with an inductively coupled argon plasma mass spectrometer (ICP MS) were used to purify the As-BP in hamster plasma. SDS-PAGE was used to confirm the arsenic-binding proteins at each purification step.</p><p><b>RESULTS</b>The three-step purification process successfully separated As-BP from other proteins (ie, arsenic unbound proteins) in hamster plasma. The molecular mass of purified As-BP in plasma was approximately 40-50 kD on SDS-PAGE.</p><p><b>CONCLUSION</b>The three-step purification method is a simple and fast approach to purify the As-BP in plasma samples.</p>