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1.
Braz. j. med. biol. res ; 32(5): 601-9, May 1999.
Artículo en Inglés | LILACS | ID: lil-233478

RESUMEN

All blood cells are derived from a small common pool of totipotent cells, called hematopoietic stem cells. The process is strictly regulated by the hematopoietic microenvironment, which includes stromal cells, extracellular matrix molecules and soluble regulatory factors. Several experimental in vitro assays have been developed for the study of hematopoietic differentiation, and have provided valuable information on the stroma, which includes, among other cell types, macrophages, fibroblasts, adipocytes, and endothelial cells. The composition, ontogeny, and function in physiological as well as pathological conditions of stroma are discussed


Asunto(s)
Humanos , Hematopoyesis , Células Madre Hematopoyéticas/fisiología , Células Madre Hematopoyéticas/patología , Sistema Hematopoyético , Células del Estroma/patología , Células del Estroma/fisiología
2.
Arq. bras. med. vet. zootec ; 49(5): 513-22, out. 1997. tab
Artículo en Portugués | LILACS | ID: lil-264560

RESUMEN

Um painel de anticorpos monoclonais (AcM) foi produzido contra antígenos da amostra "Oxford" do Vírus de Rinotraqueíte Infecciosa Bovina ou Herpesvírus Bovino tipo 1 (BHV-1). Foram selecionados cinco AcM pertencentes à classe IgG que, embora näo-neutralizantes, reconheceram todas as amostras de BHV-1.1 e BHV-1.2 quando testados por imunoperoxidase sobre cultivos de células infectadas e ELISA. Os AcM näo foram capazes de distinguir entre os subtipos BHV-1.1 e BHV-1.2


Asunto(s)
Bovinos , Herpesvirus Bovino 1 , Rinotraqueítis Infecciosa Bovina
3.
Braz. j. med. biol. res ; 30(2): 225-30, Feb. 1997. graf
Artículo en Inglés | LILACS | ID: lil-188430

RESUMEN

The mechanism whereby the immune system avoids self-aggression is one of the central issues of Immunology. The discovery of natural autoantibodies, mainly of IgM isotype, and of idiotypic interactions between antibodies indicates that elements of the immune system interact with self constituents and with themselves. Results of studies with soluble antibodies have indicated that the pool of circulating IgM represents the end result of a highly selective process of B cell activation and differentiation by self proteins resulting in the formation of a network. The objective of the present work was to determine the frequency of self-reacting B cells in normal mice. We were able to detect B cells that recognize self proteins present in extracts of different organs in normal adult, 2-3-month old, BALB/c and C57BL/6 mice with an ELISA spot assay. About 1 per cent of total IgM-secreting cells among small, LPS-stimulated spleen cells reacted with organ extracts, whereas among large spleen cells the frequency was 5- to 10- fold lower. Immunization induced an increase in the frequency of IgM-secreting cells. The present results provide cellular evidence for the results of studies done at the serological level. The physiological role of these self-recognizing cells, as well as their participation in autoimmune processes, remain to be established.


Asunto(s)
Ratones , Animales , Autoinmunidad/fisiología , Linfocitos B/inmunología , Inmunoglobulina M/metabolismo , Bazo/inmunología , Técnicas de Cultivo de Célula , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL
4.
Braz. j. med. biol. res ; 24(10): 1011-6, 1991. ilus, tab
Artículo en Inglés | LILACS | ID: lil-102081

RESUMEN

Wuchereria bancrofti microfilariae were isolated from blood of infected individuals and cultured in vitro under several conditions. RPMI 1640 and TC-199 media supplemented with fetal or human serum were able to support the microfilariae for periods up to 35 days at 37-C (viability > 85%). In contrast, in minimal essential medium the microfilariae did not survive for more than 48h. In culture kept at 28-C, where viability was much lower (approximately 10% on day 15), microfilariae differentiated into type IV larvae ("sausage form). The in vitro maintainance of microfilarial larval forms is particularly important in the case of W. bancrofti due to the absence of an experimental model for the disease


Asunto(s)
Humanos , Diferenciación Celular , Técnicas In Vitro , Wuchereria bancrofti/fisiología , Supervivencia Celular , Medios de Cultivo , Temperatura , Wuchereria bancrofti/aislamiento & purificación
5.
Braz. j. med. biol. res ; 24(11): 1133-5, 1991. tab
Artículo en Inglés | LILACS | ID: lil-105493

RESUMEN

Bone marrow cells from adult BALB/c mice were cultured at 37-C, with 5% CO2 in air, in RPMI 1640 medium complemented with fetal serum. The addition of IL-3 (5% of WEHI-3-conditioned medium) or E. coli lipopolysaccharides (LPS, 50 µg/ml) to the cultures stimulated cell proliferation (1.29 and 1.22-fold, respectively, relative to control culture), whereas the simultaneous addition of the two factors reduced the number of cells recovered by 38% relative those from control cultures (which were around 2.83 x 10***5 cells for each 10***6 plated cells). The frequency of blasts and cells with surface Ig presented the same pattern of variation (o.07 and 0.02%, respectively, in control cultures). The inhibitory effect of IL-3+LPS on cell proliferation was evident from the first day of culture, but more apparent on day 3. Macrophage-colony stimulating factor (M-CSF, L929-conditioned medium) and LPS each given alone stimulated proliferation but reduced it when given together. In contrast, fetal liver cells were not affected by the simultaneous addition of IL-3 and LPS or by M-CSF and LPS. The mechanism of action of the cumulative effect of these two factors in unknown. Since crude cell-conditioned medium was used as the source of IL-3, it is possible that another factor present in this medium interacts with LPS to cause the inhibitory effect on cell proliferation


Asunto(s)
Animales , Médula Ósea/citología , Escherichia coli , Técnicas In Vitro , Interleucina-3/farmacología , Lipopolisacáridos , Médula Ósea/efectos de los fármacos , División Celular/efectos de los fármacos , Medios de Cultivo , Factores de Crecimiento de Célula Hematopoyética/farmacología , Ratones Endogámicos BALB C
6.
Rev. bras. genét ; 3(4): 387-93, Dec. 1980. tab
Artículo en Inglés | LILACS | ID: lil-60991

RESUMEN

Alguns alcalóides pirrolizidínicos hepatóxicos inibem a divisäo celular no fígado de ratos, após hepatectomía parcial. A atividade antimitótica da integerrimina, um alcalóide extraido de Senecio brasiliensis Less.var.tripartitus foi testada em ratos machos de uma linhagem endocruzada. Como estímulo `a divisäo celular, foi utilizada a hepatectomia parcial. Injeçöes intraperitoneais em doses de 0,025, 0,05, 0,2 e 0,4 da DL50 de integerrimina, 40 dias antes da hepatectomia, resultaram em 72,85, 82,02, 92,25 e 94,22% de inibiçäo da mitose, respectivamente. Nos animais que receberam as doses mais altas do alcalóide, foram observadas alteraçöes megalocíticas iniciais


Asunto(s)
Ratas , Animales , Masculino , División Celular , Hepatectomía , Alcaloides de Pirrolicidina/farmacología
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