RESUMEN
Objetivos: Avaliar três protocolos de irrigação final para remoção de detritos e material obturador de istmos simulados. Métodos: Setenta e oito pré-molares foram preparados com o sistema WaveOne Gold 45.05. Os dentes foram seccionados longitudinalmente e um istmo padronizado foi confeccionado no terço apical. Seis raízes foram utilizadas como controle negativo. Os espécimes foram randomizados em dois grupos principais (n=36). Trinta e seis raízes tiveram o istmo preenchido com detritos e as outras trinta e seis raízes foram obturadas com guta-percha e cimento endodôntico, por meio da técnica híbrida de Tagger. Cada grupo foi dividido em três subgrupos (n=12), de acordo com o protocolo de irrigação final: Easy Clean; irrigação ultrassônica passiva (PUI); e XP Clean. A ativação do NaOCl 2,5% pelo Easy Clean foi realizada em três ciclos de vinte segundos; e pelo XP Clean, por um minuto em movimento reciprocante e contínuo, respectivamente. No grupo PUI, o irrigante foi ativado em três períodos de vinte segundos. Resultados: Não foi encontrada associação entre os escores de remoção de detritos e os protocolos de irrigação (p=0,165). Foi observada associação entre os escores de remoção do material obturador e os protocolos de irrigação final (p<0,05). Conclusões: Os protocolos de irrigação final foram efetivos para a remoção de detritos, mas não para remoção de material obturador de istmos no terço apical (AU).
This study aimed to assess three final irrigation protocols on debris and filling removal from simulated irregularities. Methods: Seventy-eight mandibular premolars were prepared with WaveOne Gold system 45.05, splitted longitudinally, and a standardized groove was performed in the apical portion of the canals. Six roots served as negative control. The roots were randomly divided into two main groups (n = 36). The grooves were filled with dentin debris in thirty six roots. The others thirty-six were filled with gutta-percha and endodontic sealer using Tagger hybrid technique. Each main group was then divided into three groups (n = 12) according to the final irrigation protocol: Easy Clean, passive ultrasonic irrigation (PUI), and XP Clean. In Easy Clean 2.5% NaOCl activation was performed for 3 cycles of 20 seconds and XP Clean for one minute under reciprocating and continuous rotary motion and continuous rotary motion, respectively. In the PUI group, the irrigant was activated in three periods of 20 seconds. Results: No association was found among the scores of debris removal and the irrigation protocols (p = .165). An association was observed among the filling material removal scores and irrigation protocols (p< .05). Conclusion: Easy Clean, PUI and XP Clean were effective for dentin debris removal, but did not for filling material from artificial grooves in the apical third (AU).
Asunto(s)
Diente Premolar , Microscopía Electrónica de Rastreo , Eficacia , ElevaciónRESUMEN
AIM: The aim of this study was to evaluate the efficacy of different lengths of time of passive ultrasonic irrigation (PUI) in removing calcium hydroxide (CH) paste from root canal, using scanning electron microscopy and energy dispersive spectrometry (SEM/EDS). MATERIAL AND METHODS: Fifty-four human premolars were used. After coronal access, a size #15 K-file was used to obtain apical patency. Root canal preparation was performed using WaveOne 40.08 instruments. CH paste was placed into the root canal. Specimens were stored in a humid environment, and after seven days, they were divided into five groups (n=12) according to the irrigation protocols: Manual a size #40 K-file; PUI/1 1 min; PUI/2 2 min; PUI/3 3 min; and Control without CH paste. An elementary chemical microanalysis (SEM/EDS) was performed to quantify the presence of calcium on the dentinal walls. RESULTS: The percentage of calcium was higher in all experimental groups when compared to the control group (P < 0.05); but no differences among them were found (P > 0.05). CONCLUSION: The amount of calcium hydroxide paste on the dentinal walls was not dependent on length of time of ultrasonic activation. SEM/EDS analysis seems to be a reliable method to assess CH paste removal from the root canals.
RESUMEN
AIM: The aims of this study were to assess the penetration of two endodontic sealers (salicylate and epoxy resin-based sealers) into dentinal tubules using CLSM; and to evaluate the bacterial leakage of roots filled with the same sealers associated with gutta-percha. MATERIAL AND METHODS: For sealer penetrability assessment, thirty bovine roots were instrumented and divided into three groups: AHP: EDTA + filling with AH Plus and gutta-percha (n=10), MTAF: EDTA + filling with MTA Fillapex and gutta-percha (n=10), control group: canals were not irrigated with EDTA and were filled with gutta-percha and AH Plus (n=5) or MTA Fillapex (n=5). Rhodamine B was added to the sealers in order to provide adequate fluorescence. The roots were transversely sectioned 3mm from the apex to enable CLSM analysis. Leakage was evaluated for turbidity of the broth in a split chamber model system for 30 days, using Enterococcus faecalis as a microbial marker. Thirty roots were instrumented and divided in four grupos: AHP: filling with AH Plus and gutta-percha (n=10); MTAF: filling with MTA Fillapex and gutta-percha (n=10); positive control: filling with gutta-percha without sealer (n = 5); negative control: sealing with cyanoacrylate to test the seal of the system (n = 5). RESULTS: The medians for dentinal tubule penetration were 6.8% (AHP) and 6.6% (MTAF) (P = 0.82). The average time for bacterial leakage was 8 days in both experimental groups (P = 0.79). CONCLUSION: MTA Fillapex and AH Plus presented similar behavior regarding dentinal tubule penetration and bacterial leakage.