RESUMEN
Objective: Endometriosis is a common disease in which the endometrial stroma and glands grow abnormally outside the uterine cavity. The establishment of animal models can be effective for determining the etiology of endometriosis. In this study we compare two endometriosis models using endometrial fragments and isolated cultured endometrial cells
Methods: We obtained endometrial tissues that were in the proliferative or secretory phases from women who underwent hysteroscopies for benign reasons at Imam Khomeini Hospital [Tehran]. Following confirmation of the tissue's normality, we cut the tissues into 2 mm cube pieces. The remainder of endometrial tissues were used for isolation and cultured to the fourth passage. In the first model of endometriosis, the endometrial tissue fragments and in the second model, 2×106 isolated endometrial cells were subcutaneously transplanted into gamma irradiated mice. The mice were kept under controlled, sterile conditions for 20 days. The mice sera were collected before and after transplantation for assessment of 17beta estradiol. The ectopic tissues in both models were assessed for morphological staining using hematoxylin and eosin, as well as periodic acid Schiff [PAS] for gland secretion. The gland sections per mm2 were analyzed
Results: At 20 days after tissue transplantation, we observed endometrial-like glands in the subcutaneous tissue of both endometriosis models. The number of gland sections was 57.55 +/- 17.18/mm2 for the first model and 271.57 +/- 77.98/mm2 for the second model. This result was significantly higher in the second model when compared to the first model. Gland secretion was positive for PAS. The level of 17beta estradiol was higher in both models compared to the control group. This level was significantly higher in the second model compared to the first [P
Conclusion: The endometriosis model that used cultured endometrial cells showed more efficiency in morphology, gland formation and level of17beta estradiol
RESUMEN
Objective: This study evaluates the effect of a mouse fallopian tube cell co-culture on the proliferation of human endometrial stromal cells
Methods: We used hysteroscopy to collect the endometrial cells from the uterus. The cells were isolated with collagenase type 3 and passed through 150 microm and 40 microm filters, respectively, after which the isolated cells were cultured in DMEM/F12 medium. At the end of the fourth subculture we used flow cytometry to evaluate the percentage of CD90 positive cells. The endometrial cells were co-cultured with mitomycin C-treated cells from the mouse fallopian tube as the experimental group. Those cultured without the feeder layer were considered the control group. The proliferation rate of cells in both groups were compared by the MTT assay
Results: The endometrial cells adhered to the floor of the plate after 24 hours. After 72 hours, they had a spindle shape which was similar to fibroblasts. The rate of CD90 positive cells at the fourth passage was 94.26 +/- 0.08%. The proliferation rate of the coculture experimental group was 1.1 +/- 0.02 and for the control group, it was 1.17 +/- 0.17 which was not significantly different
Conclusion: Co-culture of mouse fallopian tube cells with endometrial stem cells did not affect the proliferation rate of endometrial stem cells