RESUMEN
OBJECTIVE@#To investigate the effect of the presence or absence of corpus luteum on hormonal composition of follicular fluid (FF) from different sized follicles and their relationship to serum concentrations in dairy cows.@*METHODS@#Ovaries were collected from 30 clinically healthy adult female cows (Holstein Friesian) 4-7 years of age with clinically normal reproductive tracts after slaughtering. Blood samples were collected from the jugular vein before slaughter from each cow. The stage of the cycle in the cows was determined postmortem. The ovaries collected from per cow were classified with corpus luteum (CL(+)) and without corpus luteum (CL(-)). FF was aspirated from small (3-5 mm), medium (6-9 mm), and large (10-20 mm) follicles in CL(+) and CL(-) ovaries. Serum and FF samples were analyzed for estradiol-17β, progesterone, testosterone, T3 and T4 concentrations.@*RESULTS@#Results demonstrated that the FF concentrations of estradiol-17β, progesterone and testosterone in different sized follicles categories (small, medium and large follicles in CL(+) and CL(-) ovaries) were significantly higher (P≤0.05) when compared with the serum. The FF concentrations of estradiol-17β and testosterone in same follicle size categories in CL(+) and CL(-) ovaries were also significant (P<0.05). Indeed, concentrations of these hormones in the CL(-) ovaries were higher than those of the CL(+) ovaries. However, there was a statistically significant difference between medium and large follicles for progesterone concentration in CL(+) and CL(-) ovaries (P<0.05). There was a significant correlation between concentration of hormones in serum and FF with increased follicular diameter.@*CONCLUSIONS@#These results indicated that the levels of hormonal composition in the FF were related to follicular size and interestingly to the presence or absence of a corpus luteum. Indeed, the corpus luteum locally affects neighboring follicular compositions during the luteal phase of the estrous cycle in dairy cows.
RESUMEN
OBJECTIVE@#To determine the values of amniotic fluid (AF) progesterone and biochemical constituents and its associated placenta traits in Iranian crossbred ewes (Arkhar-Merino×Ghezel).@*METHODS@#Sixty ewes (2-5 years old, weighing 40-50 kg) were treated with controlled internal drug release induced 30 mg progesterone for 14 d and were injected with 400 IU pregnant mare serum gonadotropin at the time of CIDR withdrawal. After the detection of estrus, ewes were hand-mated. After expelling of each fetus accompanied by fetal membranes, 10 mL AF was taken from fetal sac for spectrophotometer methods (glucose, urea, creatinine, total protein, cholesterol, triglycerides, calcium and phosphorus) and radioimmunoassay (progesterone) analysis.@*RESULTS@#Results indicated that there were highly positive correlations between placental efficiency and cotyledon density (r=0.764, P0.01), except for PW (r=0.665).@*CONCLUSIONS@#In conclusion, the significant correlation between the AF biochemical and progesterone with placental traits for the above-mentioned metabolites suggests that metabolic changes in AF levels will reflect the condition in the AF.
RESUMEN
bjective: To determine the values of amniotic fluid (AF) progesterone and biochemical constituents and its associated placenta traits in Iranian crossbred ewes (Arkhar-Merino×Ghezel). Methods: Sixty ewes (2-5 years old, weighing 40-50 kg) were treated with controlled internal drug release induced 30 mg progesterone for 14 d and were injected with 400 IU pregnant mare serum gonadotropin at the time of CIDR withdrawal. After the detection of estrus, ewes were hand-mated. After expelling of each fetus accompanied by fetal membranes, 10 mL AF was taken from fetal sac for spectrophotometer methods (glucose, urea, creatinine, total protein, cholesterol, triglycerides, calcium and phosphorus) and radioimmunoassay (progesterone) analysis. Results: Results indicated that there were highly positive correlations between placental efficiency and cotyledon density (r=0.764, P0.01), except for PW (r=0.665). Conclusions: In conclusion, the significant correlation between the AF biochemical and progesterone with placental traits for the above-mentioned metabolites suggests that metabolic changes in AF levels will reflect the condition in the AF.
RESUMEN
Objective: To investigate the effect of the presence or absence of corpus luteum on hormonal composition of follicular fluid (FF) from different sized follicles and their relationship to serum concentrations in dairy cows. Methods: Ovaries were collected from 30 clinically healthy adult female cows (Holstein Friesian) 4-7 years of age with clinically normal reproductive tracts after slaughtering. Blood samples were collected from the jugular vein before slaughter from each cow. The stage of the cycle in the cows was determined postmortem. The ovaries collected from per cow were classified with corpus luteum (CL