Isolation of carcinoembryonic antigen from human bladder carcinoma

Carcinoembryonic antigen [CEA] was isolated from human bladder carcinoma for the first time. The obtained CEA was purified by a conventional procedure involving perchloric acid extraction followed by ethanol precipitation of tumor tissue and fractionation of this extract by gel filtration on Sepharose 4B and Sephadex G-200. The isolated purified CEA have been determined by radioimmuno-assay [RIA] and immunoelectrophoresis [IEP]. The CEA isolation procedure, which was described, saved a considerable amount of time and leads to a remarkable purification of CEA from bladder carcinoma