RESUMEN
Objective@#The aim of this study was to evaluate the impacts of platelet-rich plasma (PRP) and conditioned medium (CM) derived from endometrial stromal cells on mouse preantral follicle culture in a two-dimensional system to produce competent mature oocytes for fertilization. @*Methods@#In total, 240 preantral follicles were isolated from female mouse ovarian tissue and divided into four groups. The preantral follicles were isolated three times for each group and then cultured, respectively, in the presence of alpha minimum essential medium (control), PRP, CM, and PRP+CM. The in vitro growth, in vitro maturation, and cleavage percentage of the preantral follicles were investigated. Immunocytochemistry (IHC) was also conducted to monitor the meiotic progression of the oocytes. Additionally, the mRNA expression levels of the two folliculogenesis-related genes (Gdf9 and Bmp15) and two apoptosis-related genes (Bcl2 and Bax) were investigated using real-time polymerase chain reaction. @*Results@#In the PRP, CM, and PRP+CM groups, the preantral follicle maturation (evaluated by identifying polar bodies) were greater than the control group. The cleavage rate in the CM, and PRP+CM groups were also greater than the control group. IHC analysis demonstrated that in each treatment group, meiotic spindle was normal. In the PRP+CM group, the gene expression levels of Bmp15, Gdf9, and Bcl2 were greater than in the other groups. The Bax gene was more strongly expressed in the PRP and control groups than in the other groups. @*Conclusion@#Overall, the present study suggests that the combination of CM and PRP can effectively increase the growth and cleavage rate of mouse preantral follicles in vitro.
RESUMEN
Background: Evaluating the significance and the effects of plant-derived drugs on laboratory animal's fertility was recognized. There was antioxidant activity reported from Heracleum persicum [Golpar]
Objective: Current study aims to study the antioxidant effect of Golpar extracts on sperm parameters and chromatin quality in mice
Materials and Methods: Eighteen adult male mice were divided to 3 groups [10 wk old, 35 gr weight]: group1 received hydro alcoholic extract [1000 mg/kg, ip], group 2 received oil extract [200 mg/kg, ip] and group 3 serving as the sham control group that received sterile water. Finally, left cauda epididymis of each animal was dissected and sperm analysis was done accordingly. To asses sperm chromatin and DNA quality, we used aniline blue [AB], toluidine blue [TB],chromomycin A3 [CMA3] and acridine orange [AO] staining
Results: Progressive and non-progressive sperm motility were significantly increased in group 1 in comparison with group 3 [p=0.032]. There was an increasing trend in progressive sperm motility and decreasing trend in non-progressive sperm motility in group 2 in comparison with group 3, but the differences were not significant [p=0.221 and p=0.144, respectively]. According to the sperm chromatin quality, the results of TB and AO tests revealed significant differences [p=0.004, p=0.000, respectively] between those groups and showed that the extracts of Golpar cause DNA damage, but no differences can be observed between them in AB and CMA3 staining [p>0.05]
Conclusion: The results showed that Heracleum persicum extracts may improve sperm motility. Also, it has harmful effects on sperm chromatin condensation and DNA integrity in mice