Comparison between heat inactivation and DEAF trisacryl methods for the determination of urinary N-acetyl-ß- D-glucosaminidase isozyme in normal subjects and patients with glomerulonephritis.

We report a study on NAG-B isozyme determined by heat inactivation and DEAE trisacryl methods in 231 glomerulinepgriyis patients and normal subjects There was a good correlation between the NAG-B activities measured by both methods (r = 0.902). The NAG-B activity determined by DEAE trsacryl method was significantly higher than the heat inactivation method. The mean + SD NAG-B values of 58 normal Thai subjects, 69 Patients with idiopathic nephrotic syndrome and 50 with SLE were 0.30 + 0.23, 5.12 + 8.30 and 3.25 + 3.45 U/g creatinine respectively. There was no significant difference between sex and random single and 24 hour urine. The patients had a significantly higher NAG-B value than normal controls bur there was no difference between nephrotic syndrome and SLE. From our experience, NAG isozyme isozyme activity determination by this heat inactivation method is accurate, inexpensive, rapid and easy to perform, and therefore, it is a suitable procedure for NAG isozyme measurement.