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Objective:To investigate the effects of miR-195 on the migration, invasion and epithelial mesenchymal transition (EMT) of human gastric cancer cell line (HGC-27) and its mechanism.Methods:HGC-27 cells were cultured in vitro and they were randomly divided into control group, miR-195 negative control (NC) group and miR-195 mimics (mimics) group; the expressions of miR-195 and SALL4 mRNA were detected by real-time fluorescence quantitative PCR (RT-qPCR) ; The changes in cell morphology of each group were observed under a microscope; the expressions of SALL4, E-cadherin, N-cadherin and Vimentin in HGC-27 cells were detected by Western blot; MTT method was used to detect the change of HGC-27 cell survival rate; the abilities of migration and invasion in HGC-27 cells were detected by scratch test and invasion test (Transwell) ; and the targeting relationship between miR-195 and SALL4 was confirmed by double Luciferase Report.Results:Compared with those in control group and miR-195 NC group, the miR-195 expression level, apoptosis rate and E-cadherin protein expression level of HGC-27 cells in miR-195 mice group were significantly higher ( P<0.05) , the cell survival rate, scratch healing rate, invasion number, SALL4 mRNA and SALL4, N-cadherin, Vimentin protein expression levels were significantly lower ( P<0.05) , the morphology of HGC-27 cells returned to normal, and the epithelial mesenchymal behavior was significantly reduced; in addition, there was a binding site between miR-195 and SALL4 mRNA 3'UTR region, and compared with that in SALL4-3'UTR-WT + miR-195 NC group, the luciferase activity of SALL4-3'UTR-WT + miR-195 MICs group was lower ( P<0.05) . Conclusion:MiR-195 may inhibit the migration, invasion and epithelieal-mesenchymal transition of human gastric cancer cells by targetingly inhibiting the expression of SALL4.
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Objective To evaluate the efficacy of intravenous laser treatment for varicose great saphenous veins of lower limbs. Methods .Intravenous laser treatment in combination with surgical procedure was used to treat 126 cases of varicose great saphenous vein (150 limbs) in this hospital between July 2002 and May 2004. Results .The operation time was 30~50 min (mean, 45 min). A follow-up for 3~19 months (mean, 6 months) in 120 cases (142 limbs) was carried out. Out of the 142 limbs, symptoms of soreness and lacking in strength completely disappeared in 134 limbs (94.4%) and partially subsided in 8 limbs (5.6%). A complete disappearance of symptoms of skin pigmentation and swelling was achieved in 45 limbs (90.0%, 45/50) and 97 limbs (96.4%, 97/101), respectively. The varicose superficial veins vanished in all the limbs without recurrence. Complications included skin burn on the medial malleolus (2 cases) or anterior shank (2 cases), numbness and hypoesthesia on the medial shank (3 cases), and subcutaneous cord-like hard tubercles (32 cases, which were completely softened and absorbed 3 months later). Conclusions .Intravenous laser treatment is a reliable method in the treatment of varicose great saphenous veins, with simplicity of performance, good safety, little influence of normal activities, and excellent cosmetic results.