RESUMEN
The common treatment of Central giant cell granuloma is surgery. Recently non-surgical treatments have been described and their benefits may be worthy of consideration. Hence; we assessed the efficacy of oral calcium carbonate and intralesional triamcinolone injections as a viable treatment option in the management of Central giant cell granuloma (CGCG) of mandible. Methods: A total of 20 patients with CGCG were included in the present study. Complete oral intra-oral and extra-oral examination was carried out in all the patients. Radiograph investigation was carried out in all the patients. In all the patients, intralesional infiltration of triamcinolone was done.Approximately eight to ten injections were given in all the patients over a period of two weeks. Assessment of lesion both clinically and radiographically was done at successive follow-ups upto two years. All the results were recorded in Microsoft excel sheet and were analysed by SPSS software. Results: Radiographic resolution of the lesion at 2 year follow-up was seen in 18 patents while clinical resolution of the lesion was seen in 19 patients. Clinical success of the treatment was seen in 95 percent of the cases while radiographic success of the treatment was seen in 90 percent of the cases. Conclusion: Although treatment of CGCG is still controversial, intralesional steroid injections are safer and effective line of treatment of CGCG.
RESUMEN
Stanniocalcin-1 (STC1) is secreted by the variety of tissues having a major role in the regulation of calcium ions in the involuting mammary gland. The present work aims to sequence and structural characterization as well as expression profiling of STC1 gene in buffalo. Polymorphism identified in the 3-untranslated region (UTR) was analysed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) genotyping in riverine and swamp buffaloes. Expression profiling of STC1 was performed in different lactation stages of mammary gland and peripheral blood mononuclear cells to study the impact of 3'-UTR polymorphism on its expression. Different polymorphic sites were detected in the entire coding and noncoding regions of riverine and swamp buffaloes, including two INDELs. An identified polymorphic nucleotide locus A324G, having target sites for two miRNAs, namely bta-miR-2382 and bta-miR-1343, reported in cattle, was genotyped by PCR-RFLP to reveal variable allelic distribution among swamp and riverine buffaloes. Gene expression profiling across buffalo mammary tissues representing different lactation stages showed maximum expression of the STC1 gene in the involuting mammary gland. Ruminants’ specific genetic variation has been observed in STC1 and its implication in buffalo mammary gland involution as well as coregulation of gene expression throughmiRNA binding in the 3'-UTR is suggested.
RESUMEN
Two polyherbal formulations of Ayurveda viz., Chandraprabha Vati and Maha yogaraja Guggulu were evaluated for their free radical scavenging properties. Methanolic extracts of the formulations were studied in four different in vitro and ex vivo models. Total phenolic content of Chandraprabha Vati and Maha yogaraja Guggulu was found to be 5.24% and 10.74% respectively. Methanolic extracts of the formulations were good scavengers of all the radicals but there was a difference in the activity of the two formulations in different models. Chandraprabha Vati was a good scavenger of superoxide radical and Maha yogaraja Guggulu was efficient in scavenging nitric oxide (NO), while both inhibited lipid peroxidation efficiently. Free radical scavenging activity of the different extracts can be attributed to the presence of various chemical components including phenolics.