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2.
Experimental & Molecular Medicine ; : 93-99, 1998.
Artículo en Inglés | WPRIM | ID: wpr-70153

RESUMEN

A soluble factor which augments the expression of major histocompatibility complex class I (MHC I) antigens on a number of murine tumor cell lines, has been isolated from the culture supernatants of mixed lymphocyte reaction of spleen cells derived from C57B1/6, Balb/c and Swiss mice. The factor, termed MHC-augmenting factor (MHC-AF) has been partially purified by Sephadex G-100 column chromatography and reverse phase HPLC. MHC-AF activity is associated with an 18 kDa molecule. MHC-AF activity was resistant to pH 2.0 treatment and partially purified MHC-AF preparations did not have any activity in L929 cell/vesicular stomatitis virus (VSV) interferon bioassay system. Antibodies to IFN-gamma did not block the activity of MHC-AF. These results indicate that a MHC-AF distinct from IFN-gamma, is produced by mouse spleen cells undergoing a mixed lymphocyte reaction.


Asunto(s)
Ratones , Animales , Anticuerpos/farmacología , Quimotripsina/metabolismo , Quimotripsina/química , Estudio Comparativo , Concanavalina A/farmacología , Calor , Antígenos de Histocompatibilidad Clase I/metabolismo , Antígenos de Histocompatibilidad Clase I/efectos de los fármacos , Interferón gamma/farmacología , Interferón gamma/metabolismo , Interferón gamma/inmunología , Linfocitos/fisiología , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Proteínas/farmacología , Proteínas/metabolismo , Proteínas/aislamiento & purificación , Bazo/citología , Tripsina/metabolismo , Tripsina/química , Células Tumorales Cultivadas/inmunología , Células Tumorales Cultivadas/efectos de los fármacos
3.
Experimental & Molecular Medicine ; : 199-204, 1998.
Artículo en Inglés | WPRIM | ID: wpr-159769

RESUMEN

Mouse spleen cells activated in a mixed lymphocyte reaction release a soluble factor, which induces a significant proliferative response in fresh mouse spleen cells. This proliferation inducing factor (PIF) was found to be heat stable (90 degrees C for 45 min) and also resistant to trypsin or chymotrypsin treatment. By using a sizing HPLC column, the molecular weight of PIF appears to be 25 kDa. Mouse spleen cells treated with anti-thy-1 + complement lost Con-A induced proliferative responses but responded well to PIF. B cell depleted spleen cells obtained by negative selection panning, did not respond to PIF. These results indicate that B cells proliferated in response to PIF. Polymixin-B, which blocks the B cell proliferative response to LPS, did not inhibit PIF induced proliferation.


Asunto(s)
Ratones , Animales , Linfocitos B/fisiología , Linfocitos B/efectos de los fármacos , Médula Ósea/metabolismo , División Celular/fisiología , Cromatografía Líquida de Alta Presión , Quimotripsina/farmacología , Relación Dosis-Respuesta a Droga , Sustancias de Crecimiento/farmacología , Sustancias de Crecimiento/química , Calor , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Peso Molecular , Polimixina B/farmacología , Desnaturalización Proteica , Bazo/metabolismo , Timo/metabolismo , Tripsina/farmacología
4.
Experimental & Molecular Medicine ; : 129-132, 1997.
Artículo en Inglés | WPRIM | ID: wpr-123618

RESUMEN

A novel factor which augments the expression of major histocompatibility complex I (MHC augmenting factor or MHC-AF) antigens on tumor cell lines, has been isolated from the culture supernatants of human peripheral blood mononuclear cells activated by concanavalin-A. A mouse equivalent of this factor has also been isolated from the culture supernatants of mouse spleen cells activated by mitogens or in a mixed lymphocyte reaction. Mouse MHC-AF enhances the expression of class I MHC antigens on murine tumor cell lines (EL-4 and BW5147) but not on human tumor cell lines (K562 and HR-7). Human MHC-AF on the other hand enhances the MHC I expression on both human as well as murine cell lines. Interferon gamma (IFN-gamma), a cytokine also known to enhance the expression of MHC I antigens, acts in a highly species specific manner with mouse IFN-gamma augmenting the MHC I on murine tumor cell lines and human IFN-gamma augmenting the MHC I on human tumor cell lines only. These results indicate important differences in the cross species biological activities of MHC-AF and IFN-gamma, and provide additional evidence for MHC-AF being distinct from IFN-gamma.


Asunto(s)
Animales , Humanos , Ratones , Línea Celular , Línea Celular Tumoral , Mano , Antígenos de Histocompatibilidad Clase I , Interferones , Prueba de Cultivo Mixto de Linfocitos , Linfocitos , Linfocinas , Complejo Mayor de Histocompatibilidad , Mitógenos , Especificidad de la Especie , Bazo
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