RESUMEN
Background & objectives: With the current snags from the use of Artemisinin-combination therapies (ACTs) in malaria treatment in addition to fear of cross-resistance to unrelated drugs, raising the immunocompetence of individuals in malaria endemic areas by vaccination is the best approach to malaria-free world. Methods: Water-soluble cationic derivative, N, N, N-trimethylchitosan (TMC) was synthesized from chitosan. Nanoparticles of the TMC were prepared in various media [milliQ water, Na2CO3 (pH 10.92), Na2HPO4 (PBS, pH 9.01 and alhydrogel®] which were characterized as adjuvants for possible vaccine delivery. The nanoparticles were characterized for particle size, surface charge and morphology using microscopy (Phase contrast microscope and Confocal laser scanning microscope), and Malvern zetasizer Nano-ZS. Time-resolved particle size analysis was performed after one month storage of the TMC nanoparticles at 4°C. Results: The result of the study showed that PBS was the best medium that produced cationic, monodispersed and stable TMC nanoparticles of <65 nm forming a compatibly homogeneous system even upon storage. Details of the polyelectrolyte-doped nanoparticles in PBS showed clear coatings due to Sodium poly (styrene sulfonate) [PSS, MW ~70 kDa] at the periphery of the particles and a fluorescent core with some tiny central hollow cavities implying that the nanoparticles can either entrap the vaccine candidate into the hollow cavities or adsorb them unto the surface of the peripheral polyelectrolyte coatings. Interpretation & conclusion: This preliminary study established that TMC has the desired qualities for the intending antigen delivery. Further research regarding the biological activity of this TMC is indicated.
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The mechanism of diclofenac sodium release from non-disintegrating bioadhesive tablets was studied. Diclofenac sodium was embedded in Carbopol 941 to produce bioadhesive granules; which were later; incorporated into a structure-forming material; paraffin wax. The tablets were formulated by pour moulding in a well-lubricated plastic mould. The tablets were thereafter evaluated as compressed uncoated tablets using the following parameters: weight uniformity; friability; hardness; thickness; absolute drug content and release studies. The release study was undertaken in simulated intestinal fluid (SIF) without pancreatin. The result of the study showed that the release of diclofenac sodium was extended in all the tablets. The tablets conformed to pharmacopoeia specification as applied to uncoated compressed tablets. The mechanism of release of diclofenac sodium appeared to have followed different release models
Asunto(s)
DiclofenacoRESUMEN
The decimal assay for additivity (DAA) method was used to evaluate the in vitro interaction of glycine (Gly) with penicillin G (Pen G); cloxacillin (Clox) and ampicillin (Amp) against a clinical isolate of Staphylococcus aureus. In the interaction between Pen G/glycine with biological equivalent factor (BEF) 5 mg/62 mg; the decimal combination of 4 parts Pen G and 6 parts glycine showed amtagpmos; wjo;e ptjers sjpwed addotive effect In the interaction between Clox/glycine with BEF 1.26 og/62.5 mg ony 7:3 combination gave a synergistic effect. Others showed antagonism. In Amp/glycine combination with BEF; 1.74 og/62.5 mg; the decimal combinations of 4:6 gave additive effect; 9:1 gave synergistic effect while 7:3 combinaiton gave indifferent effect